Tanabe Rima, Cha Byung-Yoon, Ha Byung Geun, Yonezawa Takayuki, Nagai Kazuo, Woo Je-Tae, Yagasaki Kazumi
Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.
J Immunoassay Immunochem. 2012;33(2):166-79. doi: 10.1080/15321819.2011.615360.
We used a ProteoChip coated with a calixcrown derivative protein linker to measure adiponectin and monocyte chemoattractant protein-1 (MCP-1) levels and compared the results with commercial enzyme-linked immunosorbent assay (ELISA) kits. Adiponectin and MCP-1 levels in normal human serum and RAW264 cell supernatants, respectively, were measured. The ProteoChip quantification results correlated with those from the ELISA kits; however, the ProteoChip required less sample volume, exhibited higher sensitivity, and had a wider detection range. The ProteoChip was capable of detecting and quantifying small amounts of protein, possibly replacing ELISA kits in evaluating the levels of adiponectin and MCP-1.
我们使用涂有杯芳烃冠醚衍生物蛋白质连接体的蛋白质芯片来测量脂联素和单核细胞趋化蛋白-1（MCP-1）的水平，并将结果与商用酶联免疫吸附测定（ELISA）试剂盒进行比较。分别测定了正常人血清和RAW264细胞上清液中的脂联素和MCP-1水平。蛋白质芯片定量结果与ELISA试剂盒的结果相关；然而，蛋白质芯片所需样品体积更少，灵敏度更高，检测范围更广。该蛋白质芯片能够检测和定量少量蛋白质，可能在评估脂联素和MCP-1水平方面取代ELISA试剂盒。
