密蒙花乙醇提取物对激素依赖性人乳腺癌 MCF-7 细胞的体外细胞毒性。

Department of Nutrition and Dietetics, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor 43400, Malaysia.

BMC Complement Altern Med. 2012 Apr 4;12:35. doi: 10.1186/1472-6882-12-35.

BACKGROUND

Strobilanthes crispus has been traditionally used as antidiabetic, anticancer, diuretic, antilytic and laxative agent. However, cytotoxicity and antiproliferative effect of S. crispus is still unclear.

RESULTS

Strobilanthes cripus was able to reduce cell viability and proliferation in MTT and BrdU assays. Both cell cycle progression and Tunel assay suggested that IC50 of S. crispus ethanol extract induced sub-G1 cell cycle phase, and DNA fragmentation. On the other hand, translocation of mitochondria cytochrome c release, induction of caspase 3/7 and p53 while suppress XIAP on treated MCF-7 cell were also observed in this study.

CONCLUSION

Our findings suggest that S. crispus ethanol extract induced apoptosis and DNA fragmentation on hormone dependent breast cancer cell line MCF-7 via mitochondria dependent p53 apoptosis pathway.

背景

密蒙花在传统上被用作降血糖、抗癌、利尿、溶石和通便剂。然而，密蒙花的细胞毒性和抗增殖作用尚不清楚。

结果

密蒙花能够降低 MTT 和 BrdU 测定中的细胞活力和增殖。细胞周期进程和 Tunel 分析表明，密蒙花乙醇提取物的 IC50 诱导细胞进入亚 G1 期细胞周期，并导致 DNA 片段化。另一方面，在 MCF-7 细胞中还观察到线粒体细胞色素 c 释放的易位、caspase 3/7 和 p53 的诱导，以及 XIAP 的抑制。