Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University, Haikou 570228, China.
J Fish Biol. 2012 Apr;80(4):866-75. doi: 10.1111/j.1095-8649.2012.03230.x. Epub 2012 Mar 5.
The cytogenetics of yellow grouper Epinephelus awoara was studied using multiple cytogenetic markers [Giemsa staining, C-banding, Ag-NORs and fluorescence in situ hybridization (FISH)]. Giemsa staining results showed that the karyotypic formula of E. awoara was 2n = 48a, FN (fundamental number) = 48. Faint C-bandings were only detected at the centromeric regions of chromosome pair number 24, being almost indiscernible on the other chromosome pairs. After Ag-NOR staining, one pair of nucleolar organizer regions (NOR) was observed in the subcentromeric region of pair number 24. FISH results showed that 5S rDNA was located at a pair of medium-sized chromosomes, while 18S rDNA appeared at the same location in the subcentromeric region of pair number 24 where Ag-NORs were detected. The telomeric sequence (TTAGGG)(n) detected by FISH was located at both ends of each chromosome. The results suggested that E. awoara has retained general karyotypic structure stability during the evolutionary diversification process.
采用多种细胞遗传学标记[吉姆萨染色、C-带、Ag-NORs 和荧光原位杂交（FISH）]对黄鳍石斑鱼 Epinephelus awoara 的细胞遗传学进行了研究。吉姆萨染色结果表明，E. awoara 的核型公式为 2n = 48a，FN（基数）= 48。仅在染色体对 24 的着丝粒区域检测到微弱的 C 带，在其他染色体对上几乎无法识别。Ag-NOR 染色后，在染色体对 24 的亚着丝粒区域观察到一对核仁组织区域（NOR）。FISH 结果表明，5S rDNA 位于一对中等大小的染色体上，而 18S rDNA 则出现在 Ag-NOR 检测到的染色体对 24 的亚着丝粒区域的相同位置。通过 FISH 检测到的端粒序列（TTAGGG）（n）位于每条染色体的两端。结果表明，E. awoara 在进化多样化过程中保留了一般的核型结构稳定性。
