Santiquet Nicolas, Vallières Luc, Pothier François, Sirard Marc-André, Robert Claude, Richard François
Centre de recherche en biologie de la reproduction, Université Laval, Québec, Québec, Canada.
Cell Reprogram. 2012 Apr;14(2):123-9. doi: 10.1089/cell.2011.0066.
It is generally accepted that mammalian females are born with a finite pool of oocytes and that this is the sole source of ovules throughout the reproductive life of the adult. This dogma was shaken in 2003 when researchers showed that the oocyte stock might be renewable in adult mammals. It has been proposed that hematopoietic stem cells might be a source of new oocytes. These discoveries have puzzled many researchers and remain controversial. In our study, we attempted to determine if transplanted bone marrow cells could provide new oocytes in PU.1 mice and in severe combined immunodeficiency (SCID) mice after treatment with chemotherapeutic agents. We also examined the possibility that grafted bovine embryonic ovarian cortex might provide an environment favoring such a response. We found no evidence that transplanted bone marrow cells provide new fertilizable oocytes in PU.1 mice, in SCID mice treated with chemotherapeutic agents, or with bovine embryonic ovarian tissue grafts. However, transplanted bone marrow cells have improved the fertility of SCID mice previously treated with chemotherapeutic agents. These data suggest that bone marrow cells cannot provide new oocytes but can positively influence ovarian physiology to improve the fertility of mice previously treated with chemotherapeutic agents.
人们普遍认为，哺乳动物雌性在出生时就拥有有限的卵母细胞库，并且这是成年后整个生殖生命过程中卵子的唯一来源。2003年，当研究人员表明卵母细胞库在成年哺乳动物中可能是可再生的时，这一教条受到了动摇。有人提出造血干细胞可能是新卵母细胞的一个来源。这些发现让许多研究人员感到困惑，并且仍然存在争议。在我们的研究中，我们试图确定移植的骨髓细胞在经化疗药物处理后的PU.1小鼠和严重联合免疫缺陷（SCID）小鼠中是否能提供新的卵母细胞。我们还研究了移植的牛胚胎卵巢皮质是否可能提供有利于这种反应的环境可能性。我们没有发现证据表明移植的骨髓细胞在PU.1小鼠、经化疗药物处理的SCID小鼠或牛胚胎卵巢组织移植中能提供新的可受精卵母细胞。然而，移植的骨髓细胞提高了先前经化疗药物处理的SCID小鼠的生育能力。这些数据表明，骨髓细胞不能提供新的卵母细胞，但可以对卵巢生理产生积极影响，从而提高先前经化疗药物处理的小鼠的生育能力。
