Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Lingling Road 345, Shanghai 200032, China.
J Chromatogr A. 2012 May 18;1238:146-51. doi: 10.1016/j.chroma.2012.03.043. Epub 2012 Mar 20.
An improved CE-FA method using a polyelectrolyte multilayer (PEML) coated capillary is described. The coating was simply created by alternatively flushing the capillary with positively charged polyelectrolyte polybrene (HDB) and negatively charged polyelectrolyte dextran sulfate (DS). Human serum albumin (HSA) and 6 drugs were selected as an experimental model to evaluate such an improvement. The PEML coating was demonstrated to effectively reduce the protein adsorption on the capillary wall. Compared with the uncoated capillary, the repeatability (RSD%) of the EOF mobility and the plateau height of free drug were improved significantly from 3.7% to 0.51% and from 0.61% to 0.34%, respectively. No interference of the coating on the measurement of protein-drug binding parameters was observed.
本文描述了一种使用聚电解质多层（PEML）涂层毛细管的改进型 CE-FA 方法。该涂层通过交替冲洗带有正电荷的聚电解质多溴化联苯（HDB）和带负电荷的聚电解质硫酸葡聚糖（DS）的毛细管简单地形成。选择人血清白蛋白（HSA）和 6 种药物作为实验模型来评估这种改进。结果表明，PEML 涂层可以有效地减少蛋白质在毛细管壁上的吸附。与未涂层的毛细管相比，EOF 迁移率的重复性（RSD%）和游离药物的平台高度分别从 3.7%显著提高到 0.51%和从 0.61%提高到 0.34%。观察到涂层对蛋白质-药物结合参数测量没有干扰。
