Department of Histology and Cytophysiology, Medical University, Kilinski 1 str., 15-089 Bialystok, Poland.
Eur J Histochem. 2012 Mar 8;56(1):e10. doi: 10.4081/ejh.2012.e10.
Taking into consideration renal hypertension-induced homeostatic disorders and the key role of calcitonin gene-related peptide (CGRP) in many, systemic functions regulating systems, a question arises as to what an extent arterial hypertension affects the morphology and dynamics of pulmonary CGRP-immunopositive cell changes. The aim of the present study was to examine the distribution, morphology and dynamics of changes of CGRP-containing cells in the lungs of rats in the two-kidney, one-clip (2K1C) renovascular hypertension model. The studies were carried out on the lungs of rats after 3, 14, 28, 42, and 91 days long period from the renal artery clipping procedure. In order to identify neuroendocrine cells, immunohistochemical reaction was performed with the use of a specific antibody against CGRP. It was revealed that renovascular hypertension caused changes in the neuroendocrine, CGRP-containing cells in the lungs of rats. The changes, observed in the neuroendocrine cells, depended on time periods from experimentally induced hypertension. The highest intensity of changes in the neuroendocrine cells was observed in the lungs of rats after 14 days from the surgery.
考虑到肾性高血压引起的体内平衡紊乱，以及降钙素基因相关肽（CGRP）在许多系统功能调节系统中的关键作用，人们不禁要问，动脉高血压在多大程度上影响肺 CGRP 免疫阳性细胞变化的形态和动态。本研究的目的是研究在 2K1C 血管性高血压模型中，大鼠肺部含 CGRP 细胞的分布、形态和动态变化。研究是在肾动脉夹闭后 3、14、28、42 和 91 天的时间点进行的。为了鉴定神经内分泌细胞，使用针对 CGRP 的特异性抗体进行了免疫组织化学反应。结果表明，肾血管性高血压引起了大鼠肺部神经内分泌、含 CGRP 细胞的变化。在神经内分泌细胞中观察到的变化取决于实验性高血压后的时间间隔。在手术后 14 天，大鼠肺部的神经内分泌细胞变化最为明显。
