Ahmad Zuhaida Asra, Yeap Swee Keong, Ali Abdul Manaf, Ho Wan Yong, Alitheen Noorjahan Banu Mohamed, Hamid Muhajir
Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor, 43400 Serdang, Malaysia.
Clin Dev Immunol. 2012;2012:980250. doi: 10.1155/2012/980250. Epub 2012 Mar 15.
To date, generation of single-chain fragment variable (scFv) has become an established technique used to produce a completely functional antigen-binding fragment in bacterial systems. The advances in antibody engineering have now facilitated a more efficient and generally applicable method to produce Fv fragments. Basically, scFv antibodies produced from phage display can be genetically fused to the marker proteins, such as fluorescent proteins or alkaline phosphatase. These bifunctional proteins having both antigen-binding capacity and marker activity can be obtained from transformed bacteria and used for one-step immunodetection of biological agents. Alternatively, antibody fragments could also be applied in the construction of immunotoxins, therapeutic gene delivery, and anticancer intrabodies for therapeutic purposes. This paper provides an overview of the current studies on the principle, generation, and application of scFv. The potential of scFv in breast cancer research is also discussed in this paper.
迄今为止，单链可变片段（scFv）的产生已成为一种成熟的技术，用于在细菌系统中产生完全功能性的抗原结合片段。抗体工程的进展现已促进了一种更有效且普遍适用的方法来生产Fv片段。基本上，从噬菌体展示产生的scFv抗体可以与标记蛋白（如荧光蛋白或碱性磷酸酶）进行基因融合。这些具有抗原结合能力和标记活性的双功能蛋白可以从转化细菌中获得，并用于生物制剂的一步免疫检测。此外，抗体片段也可用于构建免疫毒素、治疗性基因递送以及用于治疗目的的抗癌胞内抗体。本文概述了目前关于scFv的原理、产生和应用的研究。本文还讨论了scFv在乳腺癌研究中的潜力。
