Kaur Pushpinder, Chaudhary Abha, Singh Bikram
Natural Plant Products Division, Institute of Himalayan Bioresource Technology (Council of Scientific and Industrial Research), Palampur-176 061, Himachal Pradesh, India.
Nat Prod Commun. 2012 Feb;7(2):203-6.
Flavonoid glycosides are a significant group of compounds found in Ginkgo biloba leaves, but the long extraction procedures in existing methods are a challenging problem. In this work, a microwave-assisted extraction (MAE) method has been developed for extracting bioactive compounds from G. biloba. Several variables were optimized, such as extracting solvent, microwave power, and extraction time that can potentially affect the extraction yield. The total phenolic content, antioxidant activity (using DPPH, ABTS and FRAP assays) and flavonoid glycosides of different extracts using RP-HPLC were assessed. The antioxidant capacity was found to be highest with MAE using 60% aq. ethanol as extracting solvent and microwave power of 120W for 20 min.
黄酮苷是银杏叶中发现的一类重要化合物，但现有方法中较长的提取过程是一个具有挑战性的问题。在这项工作中，开发了一种微波辅助提取（MAE）方法用于从银杏中提取生物活性化合物。对几个可能影响提取率的变量进行了优化，如提取溶剂、微波功率和提取时间。使用RP-HPLC评估了不同提取物的总酚含量、抗氧化活性（使用DPPH、ABTS和FRAP测定法）和黄酮苷。结果发现，使用60%乙醇水溶液作为提取溶剂、微波功率为120W、提取20分钟的微波辅助提取法的抗氧化能力最高。
