CMTM5对裸鼠移植人前列腺癌的抑制作用

[Inhibitory effect of CMTM5 on xenografted human prostatic cancer in nude mice].

作者信息

Xiao Yun-Bei, Xie Jing, Zhang Gou-Xi, Li Jing, Hao Yi-Chang, Zhang Xiao-Wei, Liu Zhen-Hua, Xu Tao, Wang Xiao-Feng

机构信息

Department of Urology, People's Hospital of Peking University, Beijing 100044, China.

出版信息

Zhonghua Nan Ke Xue. 2012 Mar;18(3):195-9.

PMID:22474980

Abstract

OBJECTIVE

To investigate the inhibitory effect of CKLF-like MARVEL transmembrane domain containing 5 (CMTM5) on xenografted human prostatic cancer in nude mice and its action mechanism.

METHODS

We established a model of xenografted prostatic cancer by inoculating PC-3 cells subcutaneously into nude mice, and 3 weeks later injected CMTM5 adenovirus locally into the tumor followed by daily observation of the tumor volume and body weight of the experimental animals. All the rats were killed 2 weeks after CMTM5 injection and the tumor tissue harvested for detection of the inhibitory effect of CMTM5 on the expressions of VEGF and NF-kappaB proteins by immunohistochemistry.

RESULTS

The tumor volume was significantly smaller and body weight of the CMTM5-treated mice were (573.39 +/- 175.24) mm3 and (0.55 +/- 0.11) g, respectively, significantly decreased as compared with those of the controls ([1482.50 +/- 327.86] mm3 and [1.31 +/- 0.29] g) (P = 0.03 and P = 0.027). Immunohistochemistry showed that the expressions of VEGF and NF-kappaB were obviously down-regulated in the CMTM5 group in comparison with the control group.

CONCLUSION

CMTM5 suppresses the growth of prostate cancer by down-regulating the expressions of VEGF and NF-kappaB.

摘要

目的

探讨含CKLF样MARVEL跨膜结构域5（CMTM5）对裸鼠人前列腺癌移植瘤的抑制作用及其作用机制。

方法

将PC-3细胞皮下接种于裸鼠建立前列腺癌移植瘤模型，3周后将CMTM5腺病毒局部注射到肿瘤内，随后每天观察实验动物的肿瘤体积和体重。注射CMTM5后2周处死所有大鼠，取肿瘤组织，通过免疫组化检测CMTM5对VEGF和NF-κB蛋白表达的抑制作用。

结果

CMTM5处理组小鼠的肿瘤体积明显较小，体重分别为（573.39±175.24）mm³和（0.55±0.11）g，与对照组（[1482.50±327.86]mm³和[1.31±0.29]g）相比显著降低（P = 0.03和P = 0.027）。免疫组化显示，与对照组相比，CMTM5组VEGF和NF-κB的表达明显下调。