Paulitsch-Fuchs A H, Fuchs E C, Wexler A D, Freund F T, Rothschild L J, Cherukupally A, Euverink G J W
Wetsus, Centre of Excellence for Sustainable Water Technology, Leeuwarden, The Netherlands.
Phys Biol. 2012 Apr;9(2):026006. doi: 10.1088/1478-3975/9/2/026006. Epub 2012 Apr 4.
When a high-voltage direct-current is applied to two beakers filled with water, a horizontal electrohydrodynamic (EHD) bridge forms between the two beakers. In this work we study the transport and behavior of bacterial cells added to an EHD bridge set-up. Organisms were added to one or to both beakers, and the transport of the cells through the bridge was monitored using optical and microbiological techniques. It is shown that Escherichia coli top10 (Invitrogen, Carlsbad, CA, USA) and bioluminescent E. coli YMC10 with a plasmid (pJE202) containing Vibrio fischeri genes can survive the exposure to an EHD liquid bridge set-up and the cells are drawn toward the anode due to their negative surface charge. Dielectrophoresis and hydrostatic forces are likely to be the cause for their transport in the opposite direction which was observed as well, but to a much lesser extent. Most E. coli YMC10 bacteria which passed the EHD bridge exhibited increased luminescent activity after 24 h. This can be explained by two likely mechanisms: nutrient limitation in the heavier inoculated vials and a 'survival of the strongest' mechanism.
当向两个装有水的烧杯施加高压直流电时，两个烧杯之间会形成一个水平的电流体动力学（EHD）桥。在这项工作中，我们研究了添加到EHD桥装置中的细菌细胞的运输和行为。将生物体添加到一个或两个烧杯中，并使用光学和微生物技术监测细胞通过桥的运输。结果表明，大肠杆菌top10（美国加利福尼亚州卡尔斯巴德市英杰公司）和带有含有费氏弧菌基因的质粒（pJE202）的发光大肠杆菌YMC10能够在暴露于EHD液桥装置后存活，并且由于其表面带负电荷，细胞被吸引向阳极。介电泳和静水力可能也是观察到的细胞向相反方向运输的原因，但程度要小得多。大多数通过EHD桥的大肠杆菌YMC10细菌在24小时后发光活性增强。这可以通过两种可能的机制来解释：接种量较大的小瓶中营养物质的限制以及“最强者生存”机制。
