School of Medical Science and Laborarory Medicine, Jiangsu University, Zhenjiang, 212013, People's Republic of China.
Cytotechnology. 2012 Dec;64(6):711-8. doi: 10.1007/s10616-012-9451-4. Epub 2012 Apr 4.
Open reading frame 17 (Bm17) gene of Bombyx mori nucleopolyhedrovirus is a highly conserved gene in lepidopteran nucleopolyhedroviruses, but its function remains unknown. In this report, transient-expression and superinfection assays indicated that BM17 localized in the nucleus and cytoplasm of infected BmN cells. To determine the role of Bm17 in baculovirus life cycle, we constructed a Bm17 knockout virus and characterized its properties in cells. Analysis of the production and infection of budded virions, the level of viral DNA replication revealed showed that there was no significant difference among the mutant, the control, and the Bm17 repaired virus strains. These results suggest that BM17 is not essential for virus replication in cultured cells.
家蚕核多角体病毒的开放阅读框 17（Bm17）基因是鳞翅目核多角体病毒中高度保守的基因，但它的功能尚不清楚。在本报告中，瞬时表达和超感染实验表明，BM17 定位于感染的 BmN 细胞的核和细胞质中。为了确定 Bm17 在杆状病毒生活周期中的作用，我们构建了 Bm17 敲除病毒，并在细胞中对其特性进行了表征。对芽生病毒粒子的产生和感染、病毒 DNA 复制水平的分析表明，突变株、对照株和 Bm17 修复病毒株之间没有显著差异。这些结果表明，BM17 在家蚕细胞中对病毒复制不是必需的。
