Institute of Inorganic and Analytical Chemistry, Westfälische Wilhelms-Universität Münster, Corrensstr. 30, 48149 Münster, Germany.
Anal Bioanal Chem. 2012 Jun;403(6):1501-22. doi: 10.1007/s00216-012-5915-9. Epub 2012 Apr 6.
Method development and applications of hyphenated techniques as tools for speciation analysis of metal-based pharmaceuticals are summarized within this review. Advantages and limitations of the separation modes-high-performance liquid chromatography (HPLC), capillary electrophoresis (CE), and gas chromatography (GC)-as well as the detection modes-inductively coupled plasma-mass spectrometry (ICP-MS) and electrospray ionization-mass spectrometry (ESI-MS)-are discussed. ICP-MS detection is found to be advantageous for the quantification of drugs containing metals and other heteroatoms. The species-independent sensitivity and multielement capabilities of ICP-MS allow it to be used for quantification even when species-specific standards are not available, as well as to determine the stoichiometry in metallodrug-biomolecule interactions. Molecular information that is totally destroyed when ICP is applied as ionization source and is therefore not obtainable via ICP-MS detection can be accessed by the complementary technique of ESI-MS. Speciation analysis combining both elemental and molecular information is therefore a powerful tool for the analysis of metal-based pharmaceuticals and their metabolites in body fluids and other relevant matrices.
本文综述了键合技术在金属基药物形态分析中的方法开发和应用。讨论了分离模式（高效液相色谱（HPLC）、毛细管电泳（CE）和气相色谱（GC））和检测模式（电感耦合等离子体质谱（ICP-MS）和电喷雾电离质谱（ESI-MS））的优缺点。发现 ICP-MS 检测有利于含金属和其他杂原子的药物的定量。ICP-MS 的物种非依赖性灵敏度和多元素能力使其即使在没有物种特异性标准品的情况下也可用于定量，并且还可确定金属药物-生物分子相互作用中的化学计量比。当 ICP 用作电离源时会完全破坏分子信息，因此无法通过 ICP-MS 检测获得的分子信息，可以通过补充的 ESI-MS 技术获得。因此，结合元素和分子信息的形态分析是分析体液和其他相关基质中金属基药物及其代谢物的有力工具。
