水牛（Bubalus bubalis）MHC-DRB cDNA 的分子特征。

Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis).

机构信息

Animal Genetics Division, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh, India.

出版信息

Genet Mol Biol. 2012 Jan;35(1):95-8. doi: 10.1590/s1415-47572012005000013. Epub 2012 Feb 2.

DOI:10.1590/s1415-47572012005000013

PMID:22481880

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3313522/

Abstract

In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB30101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β(1) domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRBA.

摘要

在本研究中，水牛 MHC（Bubu）-DRB cDNA 被克隆并进行了特征分析。扩增出的 1022 个碱基长的 cDNA 产物包含一个 801 个碱基的单一开放阅读框，编码 266 个氨基酸。Bubu-DRB 序列与牛的 BoLA-DRB30101 等位基因具有最大的同源性。共发现 7 个氨基酸残基是 Bubu-DRB 序列所特有的。大多数氨基酸取代发生在β(1)结构域。与重要功能相关的残基大多是保守的。水牛 DRB 在系统发生上与山羊 DRBA 更为接近。