[C-erbB2短发夹RNA质粒转染结肠癌细胞的生物学行为]

[Biological behaviour of colon cancer cells transfected with C-erbB2 shRNA plasmid].

作者信息

Zhao Dong-Li, Dang Cheng-Xue, Sui Yan-Xia

机构信息

Department of Oncology, First Affiliated Hospital, Medical College of Xi'an Jiaotong University, Xi'an 710061, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2012 Apr;28(4):371-3.

PMID:22482406

Abstract

AIM

To study the changes of the cell growth, cell cycle distribution and cell apoptosis of colon cancer cell, HT-29, when C-erbB2 gene was knockdown by shRNA against C-erbB2.

METHODS

Cell growth, cell cycle distribution and cell apoptosis were compared among three groups including plasmid experimental group(PEG), transfected reagent control group(TRCG)and negative plasmid control group(NPCG). Cell growth was measured by MTT assay. Cell cycle distribution and cell apoptosis were detected by flow cytometry.

RESULTS

The inhibition rate of cell growth of PEG, TRCG and NPCG were 39.65%, 7.23% and 8.05% respectively. The cell growth was significantly inhibited in PEG(P<0.01). The cells of G0/G1 phase were 74.93%, 67.19%, 68.05% respectively in PEG, TRCG and NPCG. The cells of G0/G1 phase in PEG were significantly more than those in TRCG and NPCG(P<0.05).While the cells of S phase were 7.81%, 14.02%, 13.70% in PEG, TRCG and NPCG respectively. The cells of S phase in PEG were significantly less than those in TRCG or NPCG(P<0.05).The cell apoptosis rate were 19.21%, 3.13%, 4.08% in PEG, TRCG and NPCG respectively. The cell apoptosis rate in PEG was significantly higher than those in TRCG or NPCG(P<0.01).

CONCLUSION

Cell growth was inhibited by shRNA against C-erbB2 gene. Cell cycle was blocked in G0/G1 phase and apoptosis was induced by C-erbB2 shRNA. This indicates C-erbB2 gene plays important roles in the carcinogenesis and development of colon cancer.

摘要

目的

研究针对C-erbB2基因的短发夹RNA（shRNA）敲低C-erbB2基因时，结肠癌细胞HT-29的细胞生长、细胞周期分布及细胞凋亡的变化。

方法

比较质粒实验组（PEG）、转染试剂对照组（TRCG）和阴性质粒对照组（NPCG）三组的细胞生长、细胞周期分布及细胞凋亡情况。采用MTT法检测细胞生长。通过流式细胞术检测细胞周期分布和细胞凋亡。

结果

PEG组、TRCG组和NPCG组的细胞生长抑制率分别为39.65%、7.23%和8.05%。PEG组细胞生长受到显著抑制（P<0.01）。PEG组、TRCG组和NPCG组G0/G1期细胞分别为74.93%、67.19%、68.05%。PEG组G0/G1期细胞显著多于TRCG组和NPCG组（P<0.05）。而PEG组、TRCG组和NPCG组S期细胞分别为7.81%、14.02%、13.70%。PEG组S期细胞显著少于TRCG组或NPCG组（P<0.05）。PEG组、TRCG组和NPCG组的细胞凋亡率分别为19.21%、3.13%、4.08%。PEG组细胞凋亡率显著高于TRCG组或NPCG组（P<0.01）。