Anal Chem. 2012 May 15;84(10):4262-6. doi: 10.1021/ac300761n. Epub 2012 Apr 12.
We developed a simple, compact microfluidic device to perform high dynamic-range digital polymerase chain reaction (dPCR) in an array of isolated 36-femtoliter microreactors. The density of the microreactors exceeded 20000/mm(2). This device, made from polydimethylsiloxane (PDMS), allows the samples to be loaded into all microreactors simultaneously. The microreactors are completely sealed through the deformation of a PDMS membrane. The small volume of the microreactors ensures a compact device with high reaction efficiency and low reagent and sample consumption. Future potential applications of this platform include multicolor dPCR and massively parallel dPCR for next generation sequencing library preparation.
我们开发了一种简单、紧凑的微流控装置，可在一系列隔离的 36 飞升微型反应器中进行高动态范围的数字聚合酶链反应（dPCR）。微反应器的密度超过 20000/mm(2)。该装置由聚二甲基硅氧烷（PDMS）制成，可同时将样品加载到所有微反应器中。微反应器通过 PDMS 膜的变形完全密封。微反应器的小体积确保了具有高效反应、低试剂和样品消耗的紧凑型设备。该平台的未来潜在应用包括用于下一代测序文库制备的多色 dPCR 和大规模并行 dPCR。
