State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Science, Chinese Academy of Sciences, Beijing 100085, China.
J Chromatogr A. 2012 May 18;1238:38-45. doi: 10.1016/j.chroma.2012.03.056. Epub 2012 Mar 23.
In this work, phosphatidylcholine (PC) was coated on magnetic nanoparticles to form lipid bilayer as solid-phase extraction (SPE) sorbents for the enrichment of polycyclic aromatic hydrocarbons (PAHs) from environmental water and milk samples. The lipid bilayer was coated on Fe(3)O(4) nanoparticles using a modified dry lipid film hydration method. The resulted Fe(3)O(4)/PC could be readily isolated from solution with a magnet, and exhibited excellent adsorption performance to organic pollutants. Only 0.1g of sorbents was enough to extract PAHs from 500 mL aqueous solution, and 6 mL of acetonitrile was required to desorb them. The method was fast and relied on 10 min extraction time and 5 min magnetic separation. The proposed method was successfully applied to determine PAHs in some environmental water and milk samples. The detection limit was in the range of 0.2-0.6 ng L(-1). The recoveries of the spiked water samples ranged from 89% to 115% with relative standard deviations (RSD) varying from 1% to 8%. For spiked milk samples, RSD was satisfactory (1-9%), but the recoveries were relatively low (42-62%). We show the potentials of Fe(3)O(4)/PC sorbents in environmental water and biological sample analyses.
在这项工作中，我们将磷脂酰胆碱（PC）涂覆在磁性纳米颗粒上，形成脂质双层作为固相萃取（SPE）吸附剂，用于从环境水样和牛奶样品中富集多环芳烃（PAHs）。采用改良的干脂质膜水合方法将脂质双层涂覆在 Fe(3)O(4)纳米颗粒上。所得的 Fe(3)O(4)/PC 可以很容易地用磁铁从溶液中分离出来，并表现出对有机污染物的优异吸附性能。仅需 0.1g 的吸附剂即可从 500mL 水溶液中提取 PAHs，需要 6mL 乙腈进行洗脱。该方法快速，仅需 10 分钟提取时间和 5 分钟磁分离。该方法成功应用于测定一些环境水样和牛奶样品中的 PAHs。检测限范围为 0.2-0.6ng L(-1)。水样的加标回收率在 89%至 115%之间，相对标准偏差（RSD）在 1%至 8%之间变化。对于加标牛奶样品，RSD 令人满意（1-9%），但回收率相对较低（42-62%）。我们展示了 Fe(3)O(4)/PC 吸附剂在环境水样和生物样品分析中的潜力。
