Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, China.
Anal Chim Acta. 2012 Apr 29;724:61-6. doi: 10.1016/j.aca.2012.02.046. Epub 2012 Mar 5.
In this study, a porous polypropylene frit was coated with polydimethylsiloxane (PDMS) as extraction medium, based on the home-made PDMS-frit, a rapid, simple and sensitive sorptive extraction method was established for analysis of potential biomarkers of lung cancer (hexanal and heptanal) in human serum samples. In the method, derivatization and extraction occurred simultaneously on the PDMS-frit, then the loaded frit was ultrasonically desorbed in acetonitrile. Polymerization, derivatization-extraction and desorption conditions were optimized. Under the optimal conditions, satisfactory results were gained, a wide linear application range was obtained in the range of 0.002-5.0 μmol L(-1) (R>0.997) for two aldehydes, the detection limits (SN(-1)=3) were 0.5 nmol L(-1) for hexanal and 0.4 nmol L(-1) for heptanal. The relative standard deviations (RSDs, n=5) of the method were below 7.9% and the recoveries were above 72.7% for the spiked serum. All these results hint that the proposed method is potential for disease markers analysis in complex biological samples.
在这项研究中，一种多孔聚丙烯 frit 被聚二甲基硅氧烷（PDMS）涂覆作为萃取介质，基于自制的 PDMS-frit，建立了一种快速、简单、灵敏的吸附萃取方法，用于分析人血清样品中潜在的肺癌生物标志物（己醛和庚醛）。在该方法中，PDMS-frit 上同时发生衍生化和萃取，然后将负载的 frit 在乙腈中超声解吸。优化了聚合、衍生化-萃取和洗脱条件。在最佳条件下，两种醛得到了令人满意的结果，在 0.002-5.0 μmol L(-1)（R>0.997）范围内得到了较宽的线性应用范围，对于己醛和庚醛的检测限（SN(-1)=3）分别为 0.5 nmol L(-1)和 0.4 nmol L(-1)。该方法的相对标准偏差（RSD，n=5）低于 7.9%，对于加标血清的回收率高于 72.7%。所有这些结果表明，该方法有望用于分析复杂生物样品中的疾病标志物。
