Centre for Biotechnology, Anna University, S.P road, Guindy, Chennai, Tamil Nadu 600025, India.
Microb Pathog. 2012 Jul;53(1):19-27. doi: 10.1016/j.micpath.2012.03.009. Epub 2012 Mar 30.
The effect of recombinant Brugia malayi pepsin inhibitor (rBm33) on human monocytes/macrophages has been examined using THP-1 cells. THP-1 cells stimulated with rBm33 showed enhanced levels of expression of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6) and diminished levels of IL-12, iNOS and anti-inflammatory cytokine (IL-10) expression suggesting the predominant features of Th1 response. Phorbol-12-myristate-13-acetate (PMA) treated THP-1 cells stimulated with rBm33 and subsequent incubation with GFP expressing Escherichia coli (E. coli) for 2 h enhanced the uptake of E. coli. Nitric oxide (NO) levels measured in the supernatants of these cultures did not show significant changes. Apoptotic studies with Peripheral Blood Mononuclear Cells (PBMCs) from normal individuals stimulated with rBm33 did not induce apoptosis of monocytes or lymphocytes. These observations suggest that rBm33 stimulates macrophages to induce Th1 response and does not promote apoptosis.
已使用 THP-1 细胞研究重组丝虫胃蛋白酶抑制剂（rBm33）对人单核细胞/巨噬细胞的影响。用 rBm33 刺激的 THP-1 细胞显示出促炎细胞因子（IL-1β、TNF-α、IL-6）的表达水平增强，而 IL-12、iNOS 和抗炎细胞因子（IL-10）的表达水平降低，表明 Th1 反应的主要特征。用佛波醇 12-肉豆蔻酸 13-乙酸酯（PMA）处理的 THP-1 细胞用 rBm33 刺激，随后用表达 GFP 的大肠杆菌（E. coli）孵育 2 小时，增强了 E. coli 的摄取。这些培养物上清液中测量的一氧化氮（NO）水平没有显示出显著变化。用 rBm33 刺激正常人外周血单核细胞（PBMCs）的凋亡研究没有诱导单核细胞或淋巴细胞凋亡。这些观察结果表明，rBm33 刺激巨噬细胞诱导 Th1 反应，并且不促进细胞凋亡。
