Department of Clinical and Molecular Biomedicine, Section of Pharmacology and Biochemistry, Medical School, University of Catania, Catania, Italy.
Biochem Pharmacol. 2012 Jul 1;84(1):88-92. doi: 10.1016/j.bcp.2012.03.019. Epub 2012 Mar 30.
Diabetic retinopathy is a complex disease that has potential involvement of inflammatory and oxidative stress-related pathways in its pathogenesis. We hypothesized that eriodictyol, one of the most abundant dietary flavonoids, could be effective against diabetic retinopathy, which involves significant oxidative stress and inflammation. The aim of the present study was to investigate the effects of eriodictyol in early retinal and plasma changes of streptozotocin-induced diabetic rats. The effect of eriodictyol treatment (0.1, 1, 10 mg/kg daily for 10 days) was evaluated by TNF-α, ICAM-1, VEGF, and eNOS protein levels measurement in the retina, plasma lipid peroxidation, and blood-retinal barrier (BRB) integrity. Increased amounts of cytokines, adhesion molecule, and nitric oxide synthase were observed in retina from diabetic rats. Eriodictyol treatment significantly lowered retinal TNF-α, ICAM-1, VEGF, and eNOS in a dose-dependent manner. Further, treatment with eriodictyol significantly suppressed diabetes-related lipid peroxidation, as well as the BRB breakdown. These data demonstrated that eriodictyol attenuates the degree of retinal inflammation and plasma lipid peroxidation preserving the BRB in early diabetic rats.
糖尿病性视网膜病变是一种复杂的疾病，其发病机制中可能涉及炎症和氧化应激相关途径。我们假设，桔皮素是最丰富的膳食类黄酮之一，可能对糖尿病性视网膜病变有效，因为糖尿病性视网膜病变涉及到显著的氧化应激和炎症。本研究旨在探讨桔皮素对链脲佐菌素诱导的糖尿病大鼠早期视网膜和血浆变化的影响。通过测量视网膜中 TNF-α、ICAM-1、VEGF 和 eNOS 蛋白水平、血浆脂质过氧化和血视网膜屏障（BRB）完整性，评估桔皮素治疗（0.1、1、10mg/kg，每天 10 天）的效果。糖尿病大鼠视网膜中观察到细胞因子、粘附分子和一氧化氮合酶的含量增加。桔皮素治疗以剂量依赖性方式显著降低了视网膜 TNF-α、ICAM-1、VEGF 和 eNOS。此外，桔皮素治疗还显著抑制了与糖尿病相关的脂质过氧化和 BRB 破坏。这些数据表明，桔皮素可减轻早期糖尿病大鼠视网膜炎症和血浆脂质过氧化的程度，同时保护 BRB。
