Phage Display Biotechnology Laboratory, School of Biotechnology, Institute of Agricultural Technology, Suranaree University of Technology, Thailand.
Int J Biol Macromol. 2012 Jun 1;50(5):1267-74. doi: 10.1016/j.ijbiomac.2012.03.016. Epub 2012 Mar 30.
Targeting polymers with peptides is an efficient strategy to functionalize biomaterials. Phage display technology is one of the most powerful techniques for selecting specific peptides for a wide variety of targets. A method to select a chitin-binding peptide from a 12-mer random peptide library was successfully performed against chitin immobilized in wells of microtiter plates. The synthetic chitin binding peptide (ChiBP) could bind to chitin beads and disrupt their structure. This selected peptide was successfully used to immobilize alkaline phosphatase on chitin. In addition, the peptide could induce colloidal chitin in water to form a chitin coat on the surface of plastic tubes. Scanning electron microscopy (SEM) revealed that the peptide could induce colloidal chitin and chitohexaose to form networks when the temperature was raised to 42°C.
靶向聚合物的肽是一种有效的策略，以功能化生物材料。噬菌体展示技术是选择针对各种目标的特定肽的最强大的技术之一。从固定在微孔板孔中的几丁质筛选一种 12 肽随机肽文库的方法成功地进行了。合成几丁质结合肽（ChiBP）可以结合几丁质珠并破坏其结构。该选择的肽被成功地用于将碱性磷酸酶固定在几丁质上。此外，该肽可以诱导胶体几丁质在水中形成塑料管表面的几丁质涂层。扫描电子显微镜（SEM）显示，当温度升高到 42°C 时，该肽可以诱导胶体几丁质和壳六糖形成网络。
