Department of Pharmacology, Key Laboratory of Anti-Inflammatory and Immunopharmacology, Ministry of Education, Anhui Medical University, Hefei 230032, PR China.
Food Chem Toxicol. 2012 Jun;50(6):1883-90. doi: 10.1016/j.fct.2012.03.064. Epub 2012 Mar 29.
Alzheimer's disease (AD) is a chronic neurodegenerative disorder of the elderly characterized by learning and memory impairment. Stress level glucocorticoids (GCs) and β-amyloid (Aβ) peptide deposition are found to be correlated with dementia progression in patients with AD. The astragalosides (AST) was extracted from traditional Chinese herb Astragalus membranaceous. In this study, 12 months male rats were treated with Aβ(25-35) (10 μg/rat, hippocampal CA1 injection) and dexamethasone (DEX, 1.5mg/kg, ig) and AST (8, 16 and 32 mg/kg, ig) or ginsenoside Rg1 (Rg1, 5 mg/kg, ig) for 14 days. We investigated the protective effect of AST against DEX+Aβ(25-35) injury in rats and its mechanisms of action. Our results indicate that DEX+Aβ(25-35) can induce learning and memory impairments and increase APP and Aβ(1-40) expression. AST (16, 32 mg/kg) or Rg1 (5mg/kg) treatment significantly improve learning and memory, down-regulate the mRNA levels of APP and β-secretase, decrease expression of APP and Aβ(1-40) in hippocampus. The results indicated that DEX might increase hippocampal vulnerability to Aβ(25-35) and highlight the potential neuronal protection of AST.
阿尔茨海默病（AD）是一种慢性进行性神经退行性疾病，以学习和记忆障碍为特征。研究发现，AD 患者的应激水平糖皮质激素（GCs）和β-淀粉样肽（Aβ）沉积与痴呆进展有关。黄芪甲苷（AST）是从传统中药黄芪中提取的。在这项研究中，12 个月大的雄性大鼠接受 Aβ（25-35）（10μg/大鼠，海马 CA1 注射）和地塞米松（DEX，1.5mg/kg，ig）以及 AST（8、16 和 32mg/kg，ig）或人参皂苷 Rg1（Rg1，5mg/kg，ig）治疗 14 天。我们研究了 AST 对 DEX+Aβ（25-35）损伤大鼠的保护作用及其作用机制。结果表明，DEX+Aβ（25-35）可诱导学习记忆障碍，增加 APP 和 Aβ（1-40）的表达。AST（16、32mg/kg）或 Rg1（5mg/kg）治疗可显著改善学习记忆，下调 APP 和β-分泌酶的 mRNA 水平，减少海马 APP 和 Aβ（1-40）的表达。结果表明，DEX 可能增加海马对 Aβ（25-35）的易感性，并强调了 AST 潜在的神经元保护作用。
