Department of Clinical Pathology, Faculty of Medicine, Cairo University, 23 Gezeret El Arab Street, El Mohandeseen, Cairo 12411, Egypt.
J Cancer Res Clin Oncol. 2012 Aug;138(8):1363-8. doi: 10.1007/s00432-012-1208-0. Epub 2012 Apr 7.
Diffuse large B-cell lymphoma (DLBCL) is the most common subtype of non-Hodgkin lymphoma. A number of studies have examined the role of genetic polymorphisms in the risk of DLBCL, and several variants have been identified as potential susceptibility genes, of those glutathione-S-transferases T1 and M1 (GSTT1 and GSTM1).
The aim of the current study was to investigate the influence of inherited genetic polymorphisms of GSTM1 and GSTT1 genes on the susceptibility to DLBCL in Egypt.
Genotyping of the candidate genes was performed for 71 Egyptian DLBCL patients and 100 age- and gender-matched healthy controls by multiplex polymerase chain reaction technique.
The frequencies of GSTT1 null, GSTM1 null, and dual null genotypes among DLBCL patients were 47.9, 52.1, and 23.9 % respectively.
GSTT1 null genotype conferred almost fourfold increased risk of DLBCL (OR = 3.9, 95 % CI = 1.97-7.75), and the risk increased when confined to male patients (OR = 4.4, 95 % CI = 1.57-12.63), while GSTM1 null genotype was not associated with DLBCL risk. Further studies on the functional consequences of GSTT1 and GSTM1 genetic polymorphisms would pave the way to declare their role in the pathogenesis of DLBCL or as possible predictors for response to therapy.
弥漫性大 B 细胞淋巴瘤（DLBCL）是非霍奇金淋巴瘤中最常见的亚型。许多研究已经探讨了遗传多态性在 DLBCL 风险中的作用，并且已经确定了一些变体作为潜在的易感基因，其中包括谷胱甘肽 S-转移酶 T1 和 M1（GSTT1 和 GSTM1）。
本研究旨在探讨 GSTT1 和 GSTM1 基因的遗传多态性对埃及 DLBCL 易感性的影响。
采用多重聚合酶链反应技术对 71 例埃及 DLBCL 患者和 100 例年龄和性别匹配的健康对照者进行候选基因的基因分型。
DLBCL 患者中 GSTT1 缺失、GSTM1 缺失和双重缺失基因型的频率分别为 47.9%、52.1%和 23.9%。
GSTT1 缺失基因型使 DLBCL 的发病风险增加近 4 倍（OR=3.9，95%CI=1.97-7.75），当仅限于男性患者时风险增加（OR=4.4，95%CI=1.57-12.63），而 GSTM1 缺失基因型与 DLBCL 风险无关。进一步研究 GSTT1 和 GSTM1 遗传多态性的功能后果将为阐明其在 DLBCL 发病机制中的作用或作为治疗反应的可能预测因子铺平道路。
