Lecroisey A, Keil B
Biochem J. 1979 Apr 1;179(1):53-8. doi: 10.1042/bj1790053.
The early stages of degradation of native collagen by two bacterial collagenases were studied by electron microscopy and by automatic Edman degradation. The purified collagenase from Clostridium histolyticum was shown to cleave native collagen at several sites, but not progressively from the N-terminus, as had been previously suggested. The homogeneous collagenase from Achromobacter iophagus cleaves native collagen preferentially at two sites corresponding to the interbands 33-34 and 41-42. The latter lies within the region cleaved by the eukaryotic collagenases.
通过电子显微镜和自动埃德曼降解法研究了两种细菌胶原酶对天然胶原蛋白的早期降解阶段。结果表明，来自溶组织梭菌的纯化胶原酶能在多个位点切割天然胶原蛋白，但并非如先前推测的那样从N端逐步切割。来自食菌无色杆菌的纯一胶原酶优先在对应于33 - 34和41 - 42带间区的两个位点切割天然胶原蛋白。后者位于真核生物胶原酶切割的区域内。
