Sato Ana Claudia, Andrade Sonia A, Brito Marlon V, Miranda Antonio, Sampaio Misako Uemura, de Abreu Maffei Francisco Humberto, Oliva Maria Luiza Vilela
Universidade Federal de Sao Paulo-Escola Paulista de Medicina, Departamento de Bioquimica, Rua Tres de Maio, 100, 04044-020 Sao Paulo, SP, Brazil.
Protein Pept Lett. 2012 May;19(5):501-8. doi: 10.2174/092986612800191053.
Passion fruit (Passiflora edulis Sims f. flavicarpa) is popularly known for its sedative and calming properties and is consumed as a fresh fruit or as a juice. The clinical observation of blood incoagulability associated with excessive consumption of passion fruit juice, in a patient treated with warfarin, prompted the current study to investigate in vitro the presence of blood clotting inhibitors in Passiflora edulis Sims f. flavicarpa extract. After purification process, two compounds of distinct molecular weight and inhibitory action were better characterized. One is a trypsin inhibitor similar to inhibitors from Bowman-Birk family, named PeTI-I12, and other is a compound active in coagulation that prolongs aPTT and PT, but does not change TT. The aim of this study is to provide evidence that passion fruit extract's components play a role on hemostasis and therefore may be relevant in the handling of patients treated with anticoagulants or suffering hemorrhagic diseases.
西番莲（西番莲黄果变种）以其镇静安神特性而广为人知，人们将其作为新鲜水果或果汁食用。一名接受华法林治疗的患者因过量饮用西番莲果汁出现血液凝固性降低的临床症状，促使本研究在体外研究西番莲黄果变种提取物中是否存在血液凝固抑制剂。经过纯化过程，对两种分子量和抑制作用不同的化合物进行了更深入的表征。一种是类似于鲍曼-伯克家族抑制剂的胰蛋白酶抑制剂，命名为PeTI-I12，另一种是对凝血有活性的化合物，可延长活化部分凝血活酶时间（aPTT）和凝血酶原时间（PT），但不改变凝血酶时间（TT）。本研究的目的是提供证据，证明西番莲提取物的成分在止血过程中发挥作用，因此可能与接受抗凝治疗或患有出血性疾病的患者的治疗相关。
