Dong Zhao-mei, Yao Yu-feng, Shi Lei, Tao Yu-fen, Lin Ke-qin, Huang Xiao-qin, Yang Zhao-qing, Chu Jia-you, Shi Li
Institute of Medical Biology, Chinese Academy of Medical Sciences, Kunming, Yunnan, People's Republic of China.
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2012 Apr;29(2):222-8. doi: 10.3760/cma.j.issn.1003-9406.2012.02.023.
To investigate the frequencies of HLA-Alu repeat polymorphisms (AluMICB, AluTF, AluHJ, AluHG and AluHF) in Chinese Lisu and Nu ethnic populations.
The frequencies of HLA-Alu repeat polymorphisms in above populations were determined with polymerase chain reaction (PCR). The associations between HLA-Alu repeat polymorphisms and HLA-A, HLA-B and HLA-C alleles were also analyzed. Phylogenetic trees were constructed with genetic distance calculated from the frequencies of HLA-Alu repeat polymorphisms.
Frequencies of AluTF2 and AluHF2 were different between the two populations (P< 0.05), while those of other three insertions were similar. The strength of association between HLA-Alus and HLA alleles were different (P< 0.05) in the two populations. Although AluMICB2 were associated with HLA-B56:01 in both populations, the association was stronger in Lisu population (74.0%) but moderate in Nu population (30.7%). HLA-Alus were associated with particular HLA subtypes, e.g., AluHG2 with certain HLA-A02 subtypes. By phylogenetic analysis, Lisu and Nu were clustered together with southern Chinese and Thai populations.
The distribution of HLA-Alus and the strength of associations between HLA-Alus and HLA class I alleles have varied between the two populations. Study of this association may facilitate identification of origins, evolution, progenitor haplotypes and recombination within the HLA class I region.
