Zhang Chan, Jiang Xiaochun, Chen Wanlu, Li Qi, Yun Fubin, Yang Xin, Dai Run, Cheng Yujing
Department of Blood Transfusion, The First People's Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology.
Department of Blood Transfusion, The Third People's Hospital of Yunnan Province, Kunming, Yunnan, China.
Medicine (Baltimore). 2018 Dec;97(52):e13674. doi: 10.1097/MD.0000000000013674.
Individual differences in drug clinical response are related to pharmacogenomics. The genetic variation of drug-metabolizing enzymes, drug receptors, and their downstream protein genes is the main factor causing individual differences in drug response. The genetic backgrounds among different ethnic groups are quite different. In this study, we aimed to detect the distribution difference of genotype frequency in very important pharmacogenetic (VIP) gene variants in the Lisu.Using the chi-squared test, we compared the genotype frequencies of the VIP variants in 105 Lisu people with those in 26 populations from the 1000 Genome project separately. Bonferroni's multiple adjustment was also conducted (P < .05/(26*49)). Moreover, Arlequin v3.5 and Structure v2.3.4 software were used to analyze the genetic distance and genetic structure.There were 9, 9, 11, 12, 11, 11, 9, 17, 13, 13, 16, 5, 3, 5, 3, 4, 17, 14, 16, 17, 16, 10, 13, 12, 10, and 9 single nucleotide polymorphisms that differed in frequency distribution, when Lisu people compared with the 26 populations separately. Only CYP2E1 rs2070676 was different in the Lisu population compared with the 26 groups from the 1000 Genome project. PTGS2 rs5275 and CYP2D6 rs1065852 were different in the Lisu population compared with most of the populations. Additionally, genetic backgrounds of Lisu and Han Chinese in Beijing were closest according to the lowest F-statistics value and resemblance in genetic structures.Our results complete the information of the Lisu population in pharmacogenomics database.
药物临床反应的个体差异与药物基因组学有关。药物代谢酶、药物受体及其下游蛋白基因的遗传变异是导致药物反应个体差异的主要因素。不同种族群体的遗传背景差异很大。在本研究中，我们旨在检测傈僳族中非常重要的药物遗传学（VIP）基因变异的基因型频率分布差异。我们使用卡方检验，分别将105名傈僳族人的VIP变异基因型频率与千人基因组计划中26个群体的基因型频率进行比较。还进行了Bonferroni多重校正（P <.05/(26*49)）。此外，使用Arlequin v3.5和Structure v2.3.4软件分析遗传距离和遗传结构。当傈僳族分别与26个群体比较时，有9、9、11、12、11、11、9、17、13、13、16、5、3、5、3、4、17、14、16、17、16、10、13、12、10和9个单核苷酸多态性的频率分布存在差异。与千人基因组计划的26个群体相比，只有CYP2E1 rs2070676在傈僳族群体中有所不同。与大多数群体相比，PTGS2 rs5275和CYP2D6 rs1065852在傈僳族群体中有所不同。此外，根据最低的F统计值和遗传结构的相似性，傈僳族和北京汉族的遗传背景最为接近。我们的研究结果完善了药物基因组学数据库中傈僳族群体的信息。
