Motojima K, Goto S
Department of Biochemistry, School of Pharmaceutical Sciences, Toho University, Chiba, Japan.
Biochim Biophys Acta. 1990 Nov 30;1087(3):316-22. doi: 10.1016/0167-4781(90)90005-m.
Tissue distribution of uricase (urate oxidase, EC 1.7.3.3) was studied by immunoblotting and RNA slot blot analysis. For immunoblotting, highly specific monoclonal antibodies against rat liver uricase were obtained, and for mRNA detection, a cloned uricase cDNA was used. Among seven tissues studied, uricase was immunologically detected only in the liver. The contents of uricase in other tissues, i.e., brain, thymus, heart, spleen, kidney and lactating mammary gland, were estimated to be less than 2% of that in the liver. Uricase mRNA was also detected only in the liver. The steady-state level of the mRNA in the isolated hepatocytes was relatively constant during the 8-day culture period when compared with those of other mRNAs expressed in the liver, suggesting a unique control mechanism of its expression.
通过免疫印迹法和RNA斑点印迹分析研究了尿酸酶（尿酸氧化酶，EC 1.7.3.3）的组织分布。对于免疫印迹，获得了针对大鼠肝脏尿酸酶的高度特异性单克隆抗体，对于mRNA检测，则使用了克隆的尿酸酶cDNA。在所研究的七种组织中，仅在肝脏中通过免疫方法检测到尿酸酶。其他组织，即脑、胸腺、心脏、脾脏、肾脏和泌乳乳腺中的尿酸酶含量估计不到肝脏中尿酸酶含量的2%。尿酸酶mRNA也仅在肝脏中检测到。与肝脏中表达的其他mRNA相比，分离的肝细胞中该mRNA的稳态水平在8天培养期内相对恒定，这表明其表达存在独特的调控机制。
