Zhou Hui, Sun Hong-bin, Huang Wen-bin, Xu Zheng, Su Jiang-hao, Zhu Jia-geng, Jia Rui-peng, Liu Jun
Department of Urology, First Affiliated Hospital, Nanjing Medical University, Nanjing 210006, China.
Zhonghua Yi Xue Za Zhi. 2012 Feb 28;92(8):516-9.
To analyze the stroma changes of benign and malignant human prostate tissues.
For the identification of stroma cells phenotype in human prostate cancer and benign prostate hyperplasia tissues, Masson method and immunohistochemical analysis of α-smooth muscle actin (α-SMA), desmin, and vimentin were performed. The relative volume of intratumor stroma (0%, Grade 0; 1% - 33%, Grade 1; 34% - 66%, Grade 2; 67% - 100%, Grade 3) were quantified and analyzed in local and advanced prostate cancer tissues.
Stroma myofibroblasts in prostate cancer were stained green by Masson staining and showed a co-expression of α-SMA and vimentin without an expression of desmin. It was significantly different from smooth muscle cells in benign prostate hyperplasia stained red and co-expressing a-SMA and desmin. Statistical analysis showed that high stroma volume (Grade 2/3) in advanced prostate cancer were significantly higher than that in an early stage of prostate cancer (83% vs 55%, P < 0.05).
Significant phenotypic differences of stroma cells existed in benign and malignant human prostate tissues. A high expression of myofibroblasts in advanced prostate cancer may play an important role in cancer progression. And its clinical significance should raise a high alert.
分析人前列腺良恶性组织的基质变化。
采用Masson法及α-平滑肌肌动蛋白（α-SMA）、结蛋白和波形蛋白的免疫组化分析，鉴定人前列腺癌组织和良性前列腺增生组织中的基质细胞表型。对局部和晚期前列腺癌组织中瘤内基质的相对体积（0%，0级；1% - 33%，1级；34% - 66%，2级；67% - 100%，3级）进行定量分析。
前列腺癌中的基质肌成纤维细胞经Masson染色呈绿色，显示α-SMA和波形蛋白共表达，无结蛋白表达。这与良性前列腺增生中染成红色且共表达α-SMA和结蛋白的平滑肌细胞有显著差异。统计分析显示，晚期前列腺癌中高基质体积（2/3级）显著高于前列腺癌早期（83%对55%，P < 0.05）。
人前列腺良恶性组织中基质细胞存在显著的表型差异。晚期前列腺癌中肌成纤维细胞的高表达可能在癌症进展中起重要作用。其临床意义应引起高度警惕。
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