Liu J F, Wang X, Tan H N, Liu H, Wang Y G, Chen R Q, Cao J C, Wang F S
Institute of Biochemical and Biotechnological Drugs, School of Pharmaceutical Sciences, Shandong University, Ji 'nan, Shandong, China.
Drug Discov Ther. 2010 Oct;4(5):355-61.
The effects of heparin-superoxide dismutase (SOD) conjugate (heparin-SOD) on γ-radiation induced DNA damage in vivo and in vitro were evaluated. Plasmid pcDNA3.0 solution was mixed with heparin-SOD, SOD, and a mixture of heparin and SOD (heparin + SOD), respectively, and irradiated with (60)Co at a dosage of 120 Gy. DNA injury was analyzed using agarose gel electrophoresis. The results showed that the degree of injury of pcDNA3.0 mixed with heparin-SOD, SOD, or heparin + SOD was less than that of untreated pcDNA3.0, and among them the degree of injury of pcDNA3.0 mixed with heparin-SOD was the least. It also showed that the protective effect increased with an increase of heparin-SOD concentration. The effects of SOD and heparin-SOD on the DNA damage and tumor inhibition rate of (60)Co γ-radiation exposure on tumor-bearing mice were also studied. Agarose gel electrophoresis showed that, when different SOD samples were administered before irradiation, the thymus DNA injuries of heparin-SOD, SOD, or heparin + SOD groups were more serious than that of the control group, and the DNA injuries of heparin-SOD or heparin + SOD groups were the most serious, which contradicted the above in vitro experiments. However, when heparin-SOD was administered post irradiation, it showed a repairing effect on the injured DNA.
评估了肝素-超氧化物歧化酶（SOD）共轭物（肝素-SOD）对体内和体外γ辐射诱导的DNA损伤的影响。分别将质粒pcDNA3.0溶液与肝素-SOD、SOD以及肝素和SOD的混合物（肝素+SOD）混合，并用剂量为120 Gy的⁶⁰Co进行辐照。使用琼脂糖凝胶电泳分析DNA损伤情况。结果显示，与未处理的pcDNA3.0相比，与肝素-SOD、SOD或肝素+SOD混合的pcDNA3.0的损伤程度较小，其中与肝素-SOD混合的pcDNA3.0的损伤程度最小。还表明保护作用随着肝素-SOD浓度的增加而增强。还研究了SOD和肝素-SOD对荷瘤小鼠⁶⁰Coγ辐射暴露后的DNA损伤和肿瘤抑制率的影响。琼脂糖凝胶电泳显示，在辐照前给予不同的SOD样品时，肝素-SOD、SOD或肝素+SOD组的胸腺DNA损伤比对照组更严重，且肝素-SOD或肝素+SOD组的DNA损伤最严重，这与上述体外实验结果相反。然而，在辐照后给予肝素-SOD时，它对受损DNA显示出修复作用。
