College of Life Science, China Jiliang University, Hangzhou, China.
Acta Biochim Biophys Sin (Shanghai). 2012 May;44(5):424-30. doi: 10.1093/abbs/gms018. Epub 2012 Apr 3.
MicroRNAs have been reported to be closely related to the development of human lung cancers. However, the functions of microRNAs in non-small cell lung cancer (NSCLC) remain largely undefined. Here, we investigated the role of microRNA-193b (miR-193b) in NSCLC. Our data showed that miR-193b was markedly down-regulated in NSCLC cancer tissues compared with adjacent normal tissues. The NSCLC cell line (A549) transfected with the miR-193b exhibited significantly decreased proliferation, migration, and invasion capacities when compared with the control cells. In contrast, inhibition of miR-193b increased the proliferation, migration, and invasion of A549 cells. Moreover, miR-193b repressed the expressions of cyclin D1 and urokinase-type plasminogen activator in A549 cells. These data suggest that miR-193b is a tumor suppressor in NSCLC.
microRNAs 与人类肺癌的发生发展密切相关。然而，microRNAs 在非小细胞肺癌（NSCLC）中的作用在很大程度上仍未得到明确。在这里，我们研究了 microRNA-193b（miR-193b）在 NSCLC 中的作用。我们的数据显示，与相邻正常组织相比，NSCLC 癌组织中 miR-193b 的表达明显下调。与对照细胞相比，转染 miR-193b 的 NSCLC 细胞系（A549）的增殖、迁移和侵袭能力显著降低。相反，抑制 miR-193b 则增加了 A549 细胞的增殖、迁移和侵袭。此外，miR-193b 抑制了 A549 细胞中细胞周期蛋白 D1 和尿激酶型纤溶酶原激活物的表达。这些数据表明，miR-193b 是 NSCLC 的一种肿瘤抑制因子。
