Basarslan Fatmagul, Yilmaz Nigar, Davarci Isil, Akin Mustafa, Ozgur Mustafa, Yilmaz Cahide, Ulutas Kemal Turker
Department of Pediatrics, Mustafa Kemal University, Hatay, Turkey.
Toxicol Ind Health. 2013 Sep;29(8):746-52. doi: 10.1177/0748233712442730. Epub 2012 Apr 4.
Oxidative stress is accepted as a potential responsible mechanism in the pathogenesis of radiocontrast media (RCM)-induced hepatotoxicity. Therefore, we aimed to investigate the protective effects of ebselen against RCM-induced hepatotoxicity by measuring tissue oxidant/antioxidant parameters and histological changes in rats. Wistar albino rats were randomly separated into four groups consisting of eight rats per group. Normal saline was given to the rats in control group (group 1). RCM was given to the rats in group 2, and both RCM and ebselen were given to the rats in group 3. Only ebselen was given to the rats in group 4. Liver sections of the killed animals were analyzed to measure the levels of malondialdehyde (MDA) and activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px), as well as histopathological changes. In RCM group, SOD and CAT levels were found increased. In RCM-ebselen group, MDA, SOD and CAT levels were found decreased. In RCM-ebselen group, however, GSH-Px activities of liver tissue increased. All these results indicated that ebselen produced a protective mechanism against RCM-induced hepatotoxicity and took part in oxidative stress.
氧化应激被认为是放射性造影剂（RCM）诱导的肝毒性发病机制中的一种潜在致病机制。因此，我们旨在通过测量大鼠的组织氧化/抗氧化参数和组织学变化来研究依布硒啉对RCM诱导的肝毒性的保护作用。将Wistar白化大鼠随机分为四组，每组八只。对照组（第1组）大鼠给予生理盐水。第2组大鼠给予RCM，第3组大鼠同时给予RCM和依布硒啉。第4组大鼠仅给予依布硒啉。对处死动物的肝脏切片进行分析，以测量丙二醛（MDA）水平、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）的活性，以及组织病理学变化。在RCM组中，发现SOD和CAT水平升高。在RCM-依布硒啉组中，发现MDA、SOD和CAT水平降低。然而，在RCM-依布硒啉组中，肝组织的GSH-Px活性增加。所有这些结果表明，依布硒啉对RCM诱导的肝毒性产生了保护机制，并参与了氧化应激。
