Department of Chemistry, Ochanomizu University, Otsuka, Bunkyo-ku, Tokyo, Japan.
Chem Biodivers. 2012 Apr;9(4):789-805. doi: 10.1002/cbdv.201100240.
The chemical constituents of the root extracts and the evolutionarily neutral DNA base sequences were studied for 28 samples of Ligularia duciformis, L. kongkalingensis, and L. nelumbifolia collected in Sichuan and Yunnan Provinces of China. The samples could be classified into four chemotypes (1-4). Sesquiterpenoids having eremophilane and oplopane skeletons were isolated from two (Chemotype 1) and three (Chemotype 2) samples, respectively. Two new oplopane derivatives were isolated and their structures were determined. In 18 samples, phenylpropenoids were the major components (Chemotype 3). In five samples, neither phenylpropenoids nor sesquiterpenoids were found (Chemotype 4). Despite this large chemical variety, no correlation was found between the chemotype and the morphological criteria of species identification. The analysis of the evolutionarily neutral DNA regions also indicated that the samples were not separated into distinct clades and that introgression was extensive.
对在中国四川和云南省采集的 28 份斑鸠菊属（Ligularia）的 2 种（多榔菊，匙叶斑鸠菊）和 1 种（心叶斑鸠菊）植物的根提取物的化学成分和进化中性 DNA 碱基序列进行了研究。这些样本可以分为 4 种化学型（1-4）。从两种（化学型 1）和三种（化学型 2）样本中分别分离到具有埃里莫芬烷和奥洛烷骨架的倍半萜。分离并确定了两种新的奥洛烷衍生物。在 18 个样本中，苯基丙烯类化合物是主要成分（化学型 3）。在 5 个样本中，既没有发现苯基丙烯类化合物，也没有发现倍半萜（化学型 4）。尽管存在如此大的化学多样性，但化学型与物种鉴定的形态标准之间没有相关性。对进化中性 DNA 区域的分析也表明，这些样本没有分成不同的分支，而且杂交现象很广泛。
