DFNA5 基因的甲基化在结直肠癌中经常被检测到。
Methylation of the DFNA5 gene is frequently detected in colorectal cancer.
机构信息
Gastroenterological Surgery, Showa University Fujigaoka Hospital, Aoba-ku, Yokohama, Japan.
出版信息
Anticancer Res. 2012 Apr;32(4):1319-22.
BACKGROUND
Recently, the human deafness, autosomal dominant 5 gene, DFNA5, has frequently been detected in cancer tissues. The methylation status of the DFNA5 gene in colorectal cancer was examined and was compared to the clinocopathological findings.
MATERIALS AND METHODS
Eighty-five tumor samples and corresponding normal tissues were obtained from patients with colorectal cancer who underwent surgery at our hospital. The methylation status of the DFNA5 gene in these samples was examined by quantitative methylation-specific PCR (qMSP). Subsequently, the clinicopathological findings were correlated with the methylation status of the DFNA5 gene.
RESULTS
DFNA5 gene methylation was found in 29 (34%) out of the 85 colorectal carcinomas, suggesting that it was frequently observed in colorectal cancer. A significant correlation with methylation was observed for lymphatic vessel invasion and TNM stage (p=0.0268 and p=0.0189, respectively).
CONCLUSION
DFNA5 might act as a tumor suppressor gene and DFNA5 gene methylation might play an important role in the development of colorectal cancer. Our data implicate DFNA5 gene methylation as a novel molecular biomarker in colorectal cancer.
背景
近来，常检测到人类显性遗传性耳聋 5 号基因（DFNA5）在癌组织中出现。本研究检测了结直肠癌中 DFNA5 基因的甲基化状态，并与临床病理发现进行了比较。
材料与方法
从在我院接受手术的结直肠癌患者中获得 85 例肿瘤样本及相应的正常组织。采用实时甲基化特异性 PCR（qMSP）检测这些样本中 DFNA5 基因的甲基化状态。随后，将临床病理发现与 DFNA5 基因的甲基化状态相关联。
结果
在 85 例结直肠癌中发现有 29 例（34%）DFNA5 基因发生甲基化，提示其在结直肠癌中频繁出现。DFNA5 基因甲基化与淋巴管浸润和 TNM 分期显著相关（p=0.0268 和 p=0.0189）。
结论
DFNA5 可能作为一种肿瘤抑制基因发挥作用，DFNA5 基因甲基化可能在结直肠癌的发生发展中发挥重要作用。我们的数据提示，DFNA5 基因甲基化可作为结直肠癌的一种新的分子标志物。
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