Kuntal Himani, Sharma Vinay, Daniell Henry
Bioinformation. 2012;8(6):255-9. doi: 10.6026/97320630008255. Epub 2012 Mar 31.
Simple Sequence Repeats (SSRs) or microsatellites constitute a significant portion of genomes however; their significance in organellar genomes has not been completely understood. The availability of organelle genome sequences allows us to understand the organization of SSRs in their genic and intergenic regions. In the present work, SSRs were identified and categorized in 14 mitochondrial and 22 chloroplast genomes of algal species belonging to Chlorophyta. Based on the study, it was observed that number of SSRs in non-coding region were more as compared to coding region and frequency of mononucleotides repeats were highest followed by dinucleotides in both mitochondrial and chloroplast genomes. It was also observed that maximum number of SSRs was found in genes encoding for beta subunit of RNA polymerase in chloroplast genomes and NADH dehydrogenase in mitochondrial genomes. This is the first and original report on whole genomes sequence analysis of organellar genomes of green algae.
简单序列重复（SSRs）或微卫星构成了基因组的重要部分，然而，它们在细胞器基因组中的重要性尚未完全被理解。细胞器基因组序列的可用性使我们能够了解SSRs在其基因区域和基因间区域的组织情况。在本研究中，对绿藻门藻类的14个线粒体基因组和22个叶绿体基因组中的SSRs进行了鉴定和分类。基于该研究，观察到非编码区的SSRs数量比编码区更多，并且在线粒体和叶绿体基因组中，单核苷酸重复的频率最高，其次是二核苷酸重复。还观察到，叶绿体基因组中编码RNA聚合酶β亚基的基因以及线粒体基因组中编码NADH脱氢酶的基因中发现的SSRs数量最多。这是关于绿藻细胞器基因组全基因组序列分析的首份原创报告。
