Insect Interactions Laboratory, Luiz de Queiroz College of Agriculture, University of São Paulo, Piracicaba, SP, Brazil.
Sci Rep. 2024 Jun 4;14(1):12861. doi: 10.1038/s41598-024-63584-w.
The mitochondrial genomes of D. melacanthus and D. furcatus were sequenced and used to investigate the phylogenetic relationships with 54 species of Pentatomidae. Their mitogenomes are 17,197 and 15,444 bp-long, respectively, including 13 protein-coding genes (PCGs), 2 ribosomal RNA genes, and 22/21 transfer RNA genes, with conserved gene arrangement. Leu, Lys, and Ser were the most common amino acids in their PCGs. PCGs evolutionary analysis indicated their mitogenomes are under purifying selection, and the most conserved genes are from the cytochrome complex, reinforcing their suitability as markers for molecular taxonomy. We identified 490 mtSSRs in 56 Pentatomidae species, with large variation and a positive correlation between mtSSR number and genome size. Three mtSSRs were identified in each Diceraeus species. Only the mtSSR in the nad6 (D. melacanthus) and nad4 (D. furcatus) appear to have application as molecular markers for species characterization. Phylogenetic analysis confirmed the monophyly of Pentatomidae. However, our analysis challenged the monophyly of Pentatominae and Podopinae. We also detected unexpected relationships among some tribes and genera, highlighting the complexity of the internal taxonomic structure of Pentatomidae. Both Diceraeus species were grouped in the same clade with the remaining Carpocorini analyzed.
对 D. melacanthus 和 D. furcatus 的线粒体基因组进行了测序，并将其用于与 54 种半翅目昆虫的系统发育关系研究。它们的线粒体基因组分别长 17197 和 15444bp，包含 13 个蛋白质编码基因（PCGs）、2 个核糖体 RNA 基因和 22/21 个转移 RNA 基因，具有保守的基因排列。亮氨酸、赖氨酸和丝氨酸是它们 PCGs 中最常见的氨基酸。PCGs 的进化分析表明，它们的线粒体基因组受到纯化选择的影响，最保守的基因来自细胞色素复合物，这增强了它们作为分子分类学标记的适用性。我们在 56 种半翅目昆虫中鉴定了 490 个 mtSSR，发现其数量变化很大，与基因组大小呈正相关。在每个 Diceraeus 物种中都鉴定到了 3 个 mtSSR。只有 nad6（D. melacanthus）和 nad4（D. furcatus）中的 mtSSR 似乎可以作为物种特征的分子标记。系统发育分析证实了半翅目昆虫的单系性。然而，我们的分析对 Pentatominae 和 Podopinae 的单系性提出了挑战。我们还检测到一些部落和属之间出乎意料的关系，突出了半翅目昆虫内部分类结构的复杂性。这两个 Diceraeus 物种与分析的其余 Carpocorini 一起被分组在同一个分支中。
