Instituto de Reproducción Animal Córdoba (IRAC), Córdoba, Argentina.
Theriogenology. 2012 Jul 1;78(1):165-71. doi: 10.1016/j.theriogenology.2012.01.033. Epub 2012 Apr 10.
The aim of this study was to evaluate sperm fertilization rates and in vitro embryo development rates for sexed and non-sexed semen selected using a silane-coated silica colloid method (Isolate) or Percoll. Frozen/thawed, sexed and unsexed semen samples from four Holstein bulls were randomly allocated to one of two different density gradient selection methods. Sperm quality (motility, concentration, morphology and membrane integrity) were evaluated and compared before and after sperm selection. Sperm motility and morphology improved (P < 0.005) after the sperm selection process with no differences between the two methods. For non-sexed semen, Percoll gradient increased the mean (± SEM) percentage of sperm recovered (57.3 ± 2.8) compared to Isolate (46.0 ± 1.8; P < 0.01). However, membrane integrity was higher after Isolate than Percoll (sexed semen: 41.0 ± 0.6 vs. 38.8 ± 0.8 and non-sexed semen 60.8 ± 1.6 vs. 58.8 ± 0.5; P < 0.05). The percentage of blastocysts produced was higher when either sexed or non-sexed semen was selected by Isolate (14.0 ± 1.0; 22.0 ± 1.1) than by Percoll (10.5 ± 1.5; 17.0 ± 2.1, respectively; P < 0.05). In summary, Isolate was a more effective method for the recovery of high quality sperm for in vitro fertilization embryo production.
本研究旨在评估使用硅烷涂层硅胶胶体法（Isolate）或 Percoll 对精子进行性别分离和非性别分离选择后的受精率和体外胚胎发育率。从 4 头荷斯坦公牛的冷冻/解冻精液中随机选择有性别和无性别精液样本，分别分配到两种不同的密度梯度选择方法中。在选择前后评估和比较精子质量（活力、浓度、形态和膜完整性）。选择后精子活力和形态均有所改善（P<0.005），但两种方法之间没有差异。对于非性别分离的精液，与 Isolate（46.0±1.8）相比，Percoll 梯度增加了精子回收率的平均值（±SEM）（57.3±2.8；P<0.01）。然而，Isolate 后的膜完整性高于 Percoll（有性别精液：41.0±0.6 vs. 38.8±0.8；非性别精液：60.8±1.6 vs. 58.8±0.5；P<0.05）。当使用 Isolate 对有性别或无性别精液进行选择时，产生的囊胚百分比均高于 Percoll（有性别精液：14.0±1.0；22.0±1.1）（分别为 10.5±1.5；17.0±2.1；P<0.05）。总之，Isolate 是一种更有效的回收高质量精子用于体外受精胚胎生产的方法。
