先天性免疫基因单核苷酸多态性与中耳炎

Innate immunity gene single nucleotide polymorphisms and otitis media.

作者信息

Carroll Sarah R, Zald Philip B, Soler Zachary M, Milczuk Henry A, Trune Dennis R, MacArthur Carol J

机构信息

ENT Surgical Associates, Glendale, CA, United States.

出版信息

Int J Pediatr Otorhinolaryngol. 2012 Jul;76(7):976-9. doi: 10.1016/j.ijporl.2012.03.011. Epub 2012 Apr 9.

DOI:10.1016/j.ijporl.2012.03.011

PMID:22494793

Abstract

OBJECTIVE

Toll-like receptors (TLR) activate the innate immune system. Single nucleotide polymorphisms (SNPs) in TLR genes are linked to increased susceptibility to infections. TLR4-deficient mice have increased incidence and duration of otitis media. We hypothesize that SNPs in TLR genes are more common in otitis-prone children than in children without a history of otitis media.

METHODS

Cases (n=70) included children undergoing surgery for otitis media. Control subjects (n=70) included children undergoing surgery for non-otologic indication. Genomic DNA was extracted from blood samples. RT-PCR genotyping was performed for TLR2 (rs5743708), TLR4 (rs4986790 and rs4986791), TLR9 (rs5743836 & rs187084), and CD14 (rs2569190).

RESULTS

There were no significant differences between the groups in family history, day care, smoke exposure, allergies or prevalence of the SNPs. The most common pre-op diagnosis in control subjects was obstructive sleep apnea (OSA).

CONCLUSIONS

TLR2, TLR4, TLR9 and CD14 gene SNPs were not more prevalent in otitis-prone children.

摘要

目的

Toll样受体（TLR）激活先天性免疫系统。TLR基因中的单核苷酸多态性（SNP）与感染易感性增加有关。TLR4缺陷小鼠中耳炎的发病率和病程增加。我们推测，TLR基因中的SNP在易患中耳炎的儿童中比在无中耳炎病史的儿童中更常见。

方法

病例组（n = 70）包括接受中耳炎手术的儿童。对照组（n = 70）包括因非耳科适应症接受手术的儿童。从血样中提取基因组DNA。对TLR2（rs5743708）、TLR4（rs4986790和rs4986791）、TLR9（rs5743836和rs187084）和CD14（rs2569190）进行逆转录聚合酶链反应基因分型。