Miller F, Habicht G S
Int Arch Allergy Appl Immunol. 1977;55(1-6):228-38.
Four published methods (ion-exchange chromatography, heat, molecular sieving and alcohol fractionation) for the preparation of fractions of normal sera and tissues which possess immunosuppressive activity are compared. Human and bovine serum and human, bovine and ovine tissues including spleen, placenta and thymus were investigated as sources of substances which were immunosuppressive when added to mixed lymphocyte cultures and when injected into mice 24 h before antigenic challenge. Cohn fraction IV of human serum purified by ion-exchange chromatography at pH 5 was the most convenient preparation examined. The problems of establishing the nature of or the identity between substances being studied in different laboratories are discussed and the recommendation is made that a standard assay for activity be adopted.
对已发表的四种制备具有免疫抑制活性的正常血清和组织馏分的方法（离子交换色谱法、加热法、分子筛法和乙醇分级分离法）进行了比较。研究了人血清和牛血清以及人、牛和羊的组织（包括脾脏、胎盘和胸腺），这些组织作为物质来源，当添加到混合淋巴细胞培养物中以及在抗原攻击前24小时注射到小鼠体内时具有免疫抑制作用。通过在pH 5下进行离子交换色谱法纯化的人血清科恩IV级分是所检测的最方便的制剂。讨论了在不同实验室确定所研究物质的性质或同一性的问题，并建议采用活性标准测定法。
