Department of Animal Sciences, School of Life Sciences, University of Hyderabad, Gachibowli, Hyderabad, India.
PLoS One. 2012;7(4):e31963. doi: 10.1371/journal.pone.0031963. Epub 2012 Apr 4.
An aerobic bacterium capable of breaking down the pesticide acephate (O,S-dimethyl acetyl phosphoramidothioic acid) was isolated from activated sludge collected from a pesticide manufacturing facility. A phylogenetic tree based on the 16 S rRNA gene sequence determined that the isolate lies within the Pseudomonads. The isolate was able to grow in the presence of acephate at concentrations up to 80 mM, with maximum growth at 40 mM. HPLC and LC-MS/MS analysis of spent medium from growth experiments and a resting cell assay detected the accumulation of methamidophos and acetate, suggesting initial hydrolysis of the amide linkage found between these two moieties. As expected, the rapid decline in acephate was coincident with the accumulation of methamidophos. Methamidophos concentrations were maintained over a period of days, without evidence of further metabolism or cell growth by the cultures. Considering this limitation, strains such as described in this work can promote the first step of acephate mineralization in soil microbial communities.
从农药生产设施收集的活性污泥中分离出一种能够分解农药乙酰甲胺磷（O，S-二甲基乙酰基磷酰胺硫代酸）的需氧细菌。基于 16S rRNA 基因序列确定的系统发育树表明，该分离株位于假单胞菌属内。该分离株能够在高达 80mM 的乙酰甲胺磷浓度下生长，在 40mM 时生长最大。生长实验和静止细胞测定中用过的培养基的 HPLC 和 LC-MS/MS 分析检测到甲胺磷和乙酸的积累，表明在这两个部分之间发现的酰胺键的初始水解。如预期的那样，乙酰甲胺磷的迅速下降与甲胺磷的积累同时发生。甲胺磷的浓度在几天内保持不变，没有培养物进一步代谢或细胞生长的证据。考虑到这种局限性，像这项工作中描述的菌株可以促进土壤微生物群落中乙酰甲胺磷矿化的第一步。
