LaRoche J, Bennett J, Falkowski P G
Department of Applied Science, Brookhaven National Laboratory, Upton, NY 11973.
Gene. 1990 Nov 15;95(2):165-71. doi: 10.1016/0378-1119(90)90358-x.
Three distinct clones, encoding light-harvesting chlorophyll (LHCII) proteins associated with photosystem II were isolated from a cDNA library of the unicellular marine chlorophyte, Dunaliella tertiolecta. We determined the nucleotide sequence of one of these clones, as well as the N-terminal amino acid (aa) sequences of the four mature LHCII apoproteins (24.5, 28.5, 30 and 31 kDa). The sequenced cDNA clone encoded the precursor of the 28.5-kDa apoprotein. We deduced that the transit peptide is 30 aa long and the mature protein is processed between A and V within the peptide RAAVEFYGP. Southern blots of D. tertiolecta genomic DNA indicated the presence of three to five genes. The algal aa sequence differs from higher plants mainly in the transit peptide and the N terminus. Several aa residues, highly conserved in higher plants, which are believed to play a role in chlorophyll binding, are not conserved in the chlorophytes.
从单细胞海洋绿藻杜氏盐藻（Dunaliella tertiolecta）的cDNA文库中分离出三个不同的克隆，它们编码与光系统II相关的捕光叶绿素（LHCII）蛋白。我们测定了其中一个克隆的核苷酸序列，以及四种成熟LHCII脱辅基蛋白（24.5、28.5、30和31 kDa）的N端氨基酸序列。测序的cDNA克隆编码28.5 kDa脱辅基蛋白的前体。我们推断转运肽长30个氨基酸，成熟蛋白在肽RAAVEFYGP中的A和V之间加工。杜氏盐藻基因组DNA的Southern印迹表明存在三到五个基因。藻类的氨基酸序列与高等植物的主要区别在于转运肽和N端。几个在高等植物中高度保守、被认为在叶绿素结合中起作用的氨基酸残基，在绿藻中并不保守。
