Chávez E, Zazueta C, Díaz E
Departamento de Bioquímica, Instituto Nacional de Cardiología, D. F., Mexico.
J Bioenerg Biomembr. 1990 Oct;22(5):679-89. doi: 10.1007/BF00809071.
The effect of the alkylating reagent dicyclohexylcarbodiimide (DCCD) on mitochondrial Ca2+ content was studied. The results obtained indicate that DCCD at a concentration of 100 microM induces mitochondrial Ca2+ efflux. This reaction is accompanied by an increasing energy drain on the system, stimulation of oxygen consumption, and mitochondrial swelling. These DCCD effects can be partially suppressed by supplementing the incubation medium with 1 mM phosphate. By electrophoretic analysis on polyacrylamide-sodium dodecyl sulfate, it was found that DCCD binds to a membrane component with an Mr of 20 to 29 kDa.
研究了烷基化试剂二环己基碳二亚胺（DCCD）对线粒体钙含量的影响。所得结果表明，浓度为100微摩尔的DCCD可诱导线粒体钙外流。该反应伴随着系统能量消耗增加、氧消耗刺激以及线粒体肿胀。通过在孵育培养基中添加1毫摩尔磷酸盐，这些DCCD效应可得到部分抑制。通过聚丙烯酰胺 - 十二烷基硫酸钠电泳分析发现，DCCD与分子量为20至29千道尔顿的膜成分结合。
