[Effects of angelica polysaccharides on the proliferation of mouse skeletal muscle satellite cells and the expression of stem cell factor receptor protein].

OBJECTIVE

To observe the effects of angelica polysaccharides (APS) on the proliferation of mouse skeletal muscle satellite cells (MSCs) and c-kit expression in different in vitro hematopoietic microenvironments.

METHODS

MSCs were primarily cultured. The desmin protein was examined by immunohistochemical assay five days later. The MSCs were randomly divided into 8 groups, i. e., the control group, the supernatant from cultured bone marrow stroma cells group, 100, 200, 300 microg/mL APS added in the DMEM/F12 medium experimental groups, and 100, 200, 300 microg/mL APS intervened medium groups. The effects of the proliferation activities of MSCs were detected using MTT method. The c-kit protein of the MSCs was stained by immunohistochemistry.

RESULTS

The desmin protein was positive in the isolated cultured MSCs. Results of MTT method showed the proliferation of MSCs in APS intervened medium groups was significant. The strong positive c-kit immunoreactivity existed in APS intervened medium groups. The strong positive c-kit immunoreactivity was present in the cytoplasmic of the MSCs in the DMEM/F12 medium experimental groups and the APS intervened medium groups.

CONCLUSIONS

The APS intervened MSC medium could effectively change the growth properties of MSCs, obviously promote the proliferation of MSCs and c-kit expression. The c-kit protein might play some regulative roles in the proliferation of the MSCs.