Wang Xiao-ling, Wang Tao, Wang Ya-ni
Department of Histology and Embryology, Tianjin University of Traditional Chinese Medicine, Tianjin 300193.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2011 Aug;31(8):1093-6.
To study the effects of Danggui Buxue Decoction on the hematopoietic reconstruction of mice transplanted by muscle satellite cells ( MSCs).
MSCs were procured from newly born male mice of the homologous series. The female Kunming receptor mice irradiated with 8Gy137Cs-gamma ray were randomly divided into six groups, i. e., the blank control group, the transplanted MSC group, four groups intervened by different doses of Danggui Buxue Decoction after transplanted MSC (gastrogavage by one, three, five, and ten times of clinically equivalent dose for seven days, as the DGBX 1 group, the DGBX 2 group, the DGBX 3 group, and the DGBX 4 group). After transplantation changes of splenic colony forming unit spleen (CFU-S), white blood cells (WBC), hemoglobin (Hb), and platelet (PLT) in the peripheral blood were observed in pos-transplanted 1-, 2-, and 3-week receptor mice. The 3-week survival rate was calculated. The source of hematopoietic reconstruction was identified using PCR.
The desmin protein was positive in the cultured MSCs. WBC of each transplanted MSC group obviously increased at the 2nd week (P<0.05). Hb obviously increased in the DGBX 2 group, the DGBX 3 group, and the DGBX 4 group (P<0.05). WBC and Hb obviously increased in the DGBX 3 group and the DGBX 4 group when compared with the transplanted MSC group at the 3rd week (P<0.05). The recovery of PLT was significant in the DGBX 4 group (P<0.05). Compared with the blank control group at the 2nd week, CFU-S obviously increased in the DGBX 3 group and the DGBX 4 group (P<0.05). PCR results of Y chromosome in the survived transplant mice indicated that the hematopoietic cells of reconstruction female receptor mice were originated from male donors.
The hematopoiesis of mice transplanted by muscle satellite cells could be constructed after intervention of Danggui Buxue Decoction.
研究当归补血汤对肌肉卫星细胞（MSCs）移植小鼠造血重建的影响。
从同系新生雄性小鼠获取MSCs。将经8Gy 137Cs-γ射线照射的雌性昆明受体小鼠随机分为6组，即空白对照组、MSCs移植组、MSCs移植后用不同剂量当归补血汤干预的4组（分别按临床等效剂量的1、3、5、10倍灌胃7天，依次为当归补血汤1组、当归补血汤2组、当归补血汤3组、当归补血汤4组）。观察移植后1、2、3周受体小鼠外周血脾集落形成单位脾（CFU-S）、白细胞（WBC）、血红蛋白（Hb）及血小板（PLT）的变化。计算3周生存率。采用PCR鉴定造血重建的来源。
培养的MSCs中结蛋白蛋白呈阳性。各MSCs移植组白细胞在第2周明显升高（P<0.05）。当归补血汤2组、当归补血汤3组和当归补血汤4组血红蛋白明显升高（P<0.05）。第3周时，当归补血汤3组和当归补血汤4组白细胞和血红蛋白与MSCs移植组相比明显升高（P<0.05）。当归补血汤4组血小板恢复显著（P<0.05）。与第2周空白对照组相比，当归补血汤3组和当归补血汤4组CFU-S明显升高（P<0.05）。存活移植小鼠Y染色体的PCR结果表明，重建的雌性受体小鼠造血细胞来源于雄性供体。
当归补血汤干预后可构建肌肉卫星细胞移植小鼠的造血功能。
