两种商业实时聚合酶链反应检测方法在检测重症监护患者支气管肺泡灌洗液中曲霉和卡氏肺孢子虫 DNA 中的性能。
Performance of 2 commercial real-time polymerase chain reaction assays for the detection of Aspergillus and Pneumocystis DNA in bronchoalveolar lavage fluid samples from critical care patients.
机构信息
Dipartimento di Scienze di Sanità Pubblica, Università di Modena e Reggio Emilia, Modena, Italy.
出版信息
Diagn Microbiol Infect Dis. 2012 Jun;73(2):138-43. doi: 10.1016/j.diagmicrobio.2012.03.001. Epub 2012 Apr 12.
This article investigates the performance of 2 commercial real-time polymerase chain reaction (PCR) assays, MycAssay™ Aspergillus (Myc(Asp)Assay) and MycAssay™ Pneumocystis (Myc(PCP)Assay), on the ABI 7300 platform for the detection of Aspergillus (Asp) or Pneumocystis jirovecii (Pj) DNA in bronchoalveolar lavage (BAL) samples from 20 patients. Operationally, patients enrolled were clustered into 3 groups: invasive aspergillosis group (IA, 7 patients), Pj pneumonia group (PCP, 8 patients), and negative control group (5 patients). All the IA patients were Myc(Asp)Assay positive, whereas 12 non-IA patients returned negative PCR results. Furthermore, 7 of 8 PCP patients were Myc(PCP)Assay positive, while 9 non-PCP patients were PCR negative. In conclusion, these data provide an early indication of the effectiveness of both the Myc(Asp)Assay and Myc(PCP)Assay on the ABI 7300 platform for the detection of either Asp or Pj DNA in BAL from patients with deep fungal infections.
本文研究了两种商业实时聚合酶链反应（PCR）检测试剂盒，MycAssay™ Aspergillus（Myc（Asp）Assay）和 MycAssay™ Pneumocystis（Myc（PCP）Assay），在 ABI 7300 平台上对支气管肺泡灌洗液（BAL）样本中曲霉菌（Asp）或卡氏肺孢子虫（Pj）DNA 的检测性能，该研究共纳入了 20 名患者。在操作上，入组的患者分为 3 组：侵袭性曲霉病组（IA，7 例）、卡氏肺孢子菌肺炎组（PCP，8 例）和阴性对照组（5 例）。所有 IA 患者 Myc（Asp）Assay 均为阳性，而 12 例非 IA 患者的 PCR 结果为阴性。此外，8 例 PCP 患者中有 7 例 Myc（PCP）Assay 阳性，而 9 例非 PCP 患者 PCR 结果为阴性。总之，这些数据初步表明，Myc（Asp）Assay 和 Myc（PCP）Assay 在 ABI 7300 平台上对深部真菌感染患者的 BAL 中 Asp 或 Pj DNA 的检测具有有效性。
Zotero 插件