通过聚合酶链反应检测卡氏肺孢子虫DNA并与直接显微镜检查和免疫荧光法进行比较。
Detection of Pneumocystis carinii DNA by polymerase chain reaction compared to direct microscopy and immunofluorescence.
作者信息
Galan F, Oliver J L, Roux P, Poirot J L, Bereziat G
机构信息
Laboratoire de Biochimie, CHU Saint Antoine, Paris, France.
出版信息
J Protozool. 1991 Nov-Dec;38(6):199S-200S.
The polymerase chain reaction (PCR) using published primers and probes has been compared to conventional stains and immunofluorescence for diagnosis of Pneumocystis carinii. We have screened 71 bronchoalveolar lavage (BAL) fluids from HIV-immunosuppressed patients. Of 34 samples negative by conventional stains and immunofluorescence, only one was positive by PCR. Thirty of 35 samples positive by conventional stains and immunofluorescence were also positive by PCR. One BAL sample, negative by conventional stains but positive by immunofluorescence, was negative by PCR. These data are discussed in relation to clinical and therapeutic conditions of the patients.
使用已发表的引物和探针进行的聚合酶链反应（PCR）已与传统染色和免疫荧光法进行比较，用于诊断卡氏肺孢子虫。我们对71份来自HIV免疫抑制患者的支气管肺泡灌洗（BAL）液进行了筛查。在34份经传统染色和免疫荧光法检测为阴性的样本中，只有1份经PCR检测为阳性。在35份经传统染色和免疫荧光法检测为阳性的样本中，有30份经PCR检测也为阳性。1份经传统染色检测为阴性但经免疫荧光法检测为阳性的BAL样本，经PCR检测为阴性。结合患者的临床和治疗情况对这些数据进行了讨论。
Zotero 插件