Key Laboratory of Plant Protection Resources and Pest Integrated Management, Ministry of Education, Entomological Museum, Northwest A&F University, Yangling, Shaanxi 712100, China.
Micron. 2012 Sep;43(9):978-84. doi: 10.1016/j.micron.2012.03.022. Epub 2012 Apr 4.
The ultrastructure of mature spermatozoa of the leafhopper Cicadella viridis (Linnaeus) was investigated using light and transmission electron microscopy. The spermatozoon is composed of a head containing an acrosome and an elongated nucleus, and a long tail, which consists of a flagellum. The acrosome is conical and invaginated to form a subacrosomal space, and the acrosomal contents are filled with electron-dense tubular substructures. The nucleus is linear and filled with homogeneously condensed chromatin. The centriolar adjunct is parallel to the nucleus and connects the nucleus with the mid-piece/flagellum. The flagellum is formed by a 9+9+2 axoneme, two mitochondrial derivatives and two accessory bodies. The mitochondrial derivatives with an orderly array of peripheral cristae are symmetrical. The accessory bodies are small and slightly elliptical. The end of the axoneme shows progressive loss of microtubules. Comparison of sperm ultrastructure of C. viridis with those of other Auchenorrhyncha families supports the major relationships within Cicadomorpha as (Membracoidea (Cicadoidea, Cercopoidea)).
使用光镜和透射电镜研究了绿叶蝉（Cicadella viridis）（Linnaeus）成熟精子的超微结构。精子由头部组成，头部包含顶体和伸长的核，以及长尾巴，尾巴由鞭毛组成。顶体呈圆锥形，内陷形成亚顶体腔，顶体内容物充满电子致密的管状亚结构。核呈线性，充满均匀凝聚的染色质。中心粒附属物与核平行，并将核与中段/鞭毛连接。鞭毛由 9+9+2 轴丝、两个线粒体衍生物和两个附属体组成。排列有序的外周嵴的线粒体衍生物是对称的。附属体较小，呈椭圆形。轴丝的末端显示出微管的逐渐缺失。与其他半翅目科的精子超微结构比较，绿叶蝉的支持了 Cicadomorpha 内的主要关系为（Membracoidea（Cicadoidea，Cercopoidea））。
