Shibasaki T, Gomi H, Ishimoto F, Sakai O
Second Department of Internal Medicine, Jikei University School of Medicine.
Nihon Jinzo Gakkai Shi. 1990 Aug;32(8):871-5.
Wistar rats were injected with puromycin aminonucleoside (PAN) to induce massive proteinuria and were raised on a low protein diet to determine whether the urinary protein excretion might be reduced. Although PAN nephrotic rats fed with a normal protein diet containing 20% protein (group A) did not show any reduction of their urinary protein excretion, PAN rats fed with a low protein diet containing 6% protein (group B) revealed a marked reduction at day 9 of the present study in comparison with group A. The total serum protein levels were low in groups A and B as compared to group C, the non-treated control. However, group A recovered to normal levels at day 13. In terms of the absolute amount of oral protein intake, marked differences were observed between groups B and C, but little difference was observed between groups A and C. The body weight in all three groups was decreased at 8 days after the start of this study. The serum levels of creatinine were elevated to 1.1 +/- 0.3 mg/dl and 1.0 +/- 0.2 mg/dl in groups A and B, respectively, on day 7, but abruptly recovered to normal levels by day 12. These data indicate that the decreased urinary protein excretion in group B might be dependent on the total protein volume intake, and not on the renal function nor serum levels of total protein.
给Wistar大鼠注射嘌呤霉素氨基核苷（PAN）以诱导大量蛋白尿，并给予低蛋白饮食饲养，以确定尿蛋白排泄是否会减少。虽然喂食含20%蛋白质的正常蛋白饮食的PAN肾病大鼠（A组）未显示尿蛋白排泄有任何减少，但喂食含6%蛋白质的低蛋白饮食的PAN大鼠（B组）在本研究的第9天与A组相比，尿蛋白排泄明显减少。与未处理的对照组C组相比，A组和B组的总血清蛋白水平较低。然而，A组在第13天恢复到正常水平。就口服蛋白质摄入量的绝对量而言，B组和C组之间观察到明显差异，但A组和C组之间观察到的差异很小。在本研究开始后8天，所有三组的体重均下降。在第7天，A组和B组的血清肌酐水平分别升高至1.1±0.3mg/dl和1.0±0.2mg/dl，但在第12天突然恢复到正常水平。这些数据表明，B组尿蛋白排泄减少可能取决于总蛋白摄入量，而不是肾功能或总蛋白血清水平。
