College of Food Science and Engineering, Shandong Agricultural University, Taian 271018, China.
J Sci Food Agric. 2012 Oct;92(13):2696-702. doi: 10.1002/jsfa.5690. Epub 2012 Apr 16.
Olaquindox, as one of the antimicrobial growth accelerants, is usually used in livestock production to improve feed efficiency. Due to health concerns over possible carcinogenic, mutagenic and photoallergenic effects of olaquindox on animals, the development of simple, rapid and sensitive analytical method for determination of olaquindox is crucial and necessary.
In this study, a surface molecularly imprinted polymer was prepared by a molecular imprinting technique in combination with a sol-gel process using activated silica gel as a support material. This imprinted material exhibited with good recognition and selective ability, and fast adsorption-desorption dynamics toward olaquindox. Using it as the recognition element, a new on-line molecularly imprinted solid phase extraction coupled with chemiluminescence sensor for the determination of olaquindox was developed. The factors affecting preconcentration of the analytes and sensitivity of the method were all investigated. Under the optimal condition, the linear range of the calibration graph was between 2 × 10(-8) and 1 × 10(-6) g mL(-1) , and the detection limit of this method was 7 × 10(-9) g mL(-1) . The blank chick feed samples spiked with olaquindox at 0.3, 0.9 and 1.5 µg g(-1) levels were extracted and determined by this presented method with recoveries ranging from 87% to 94%. This method was validated by high-performance liquid chromatography and the results correlated well with those obtained by both methods. Moreover, this method was quantitatively analysed with two contaminated chick feed samples.
This study will provide a sensitive and fast method for the monitoring of olaquindox residues in foods.
奥拉喹多克斯作为一种抗菌生长促进剂，通常用于畜牧业生产以提高饲料效率。由于担心奥拉喹多克斯对动物可能具有致癌、致突变和光变应性作用，因此开发简单、快速和灵敏的分析方法来测定奥拉喹多克斯至关重要。
本研究采用分子印迹技术与溶胶-凝胶过程相结合，以活化硅胶为载体材料，制备了表面分子印迹聚合物。该印迹材料对奥拉喹多克斯表现出良好的识别和选择性，以及快速的吸附-解吸动力学。将其用作识别元件，开发了一种新的在线分子印迹固相萃取-化学发光传感器，用于测定奥拉喹多克斯。研究了影响分析物预浓缩和方法灵敏度的因素。在最佳条件下，校准曲线的线性范围为 2×10(-8)至 1×10(-6) g mL(-1)，方法的检测限为 7×10(-9) g mL(-1)。用该方法提取并测定了在 0.3、0.9 和 1.5 µg g(-1)水平下添加奥拉喹多克斯的空白鸡饲料样品，回收率在 87%至 94%之间。该方法通过高效液相色谱法进行了验证，结果与两种方法的结果相关性良好。此外，还对两个受污染的鸡饲料样品进行了定量分析。
本研究将为食品中奥拉喹多克斯残留的监测提供一种灵敏、快速的方法。
