[Analyze on volatile compounds of Antrodia camphorata using HS-SPME-GC-MS].

OBJECTIVE

To analyze the volatile compounds of Antrodia camphorata in solid-state and submerged cultures.

METHODS

A headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry(GC-MS) were used to evaluate the profile of the volatile compounds.

RESULTS

49 volatile compounds were identified in A. camphorata mycelia in submerged culture, while 43 volatile compounds were identified in mycelia in solid-state culture. 1-octen-3-ol, 3-octanone, 1-octen-3-ylacetate, acetic acid octyl ester and ethanol were the main volatile compounds in A. camphorata mycelia in submerged culture, while 1-octen-3-ol, 3-octanone, 3-methyl-butyraldenhyde, gamma-podecalactone and methyl 2-furozte were the most potent key volatile compounds in mycelia in solid-state culture.

CONCLUSION

The volatile compounds in the mycelia of A. camphorata in solid-state and submerged cultures are similar but their relative contents are different.