He Zhe, Lu Zhen-Ming, Xu Hong-Yu, Shi Jing-Song, Xu Zheng-Hong
Laboratory of Pharmaceutical Engineering, School of Medicine and Pharmaceutics, Jiangnan University, Wuxi 214122, China.
Zhong Yao Cai. 2011 Nov;34(11):1722-5.
To analyze the volatile compounds of Antrodia camphorata in solid-state and submerged cultures.
A headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography-mass spectrometry(GC-MS) were used to evaluate the profile of the volatile compounds.
49 volatile compounds were identified in A. camphorata mycelia in submerged culture, while 43 volatile compounds were identified in mycelia in solid-state culture. 1-octen-3-ol, 3-octanone, 1-octen-3-ylacetate, acetic acid octyl ester and ethanol were the main volatile compounds in A. camphorata mycelia in submerged culture, while 1-octen-3-ol, 3-octanone, 3-methyl-butyraldenhyde, gamma-podecalactone and methyl 2-furozte were the most potent key volatile compounds in mycelia in solid-state culture.
The volatile compounds in the mycelia of A. camphorata in solid-state and submerged cultures are similar but their relative contents are different.
分析固态培养和液体深层培养条件下樟芝的挥发性化合物。
采用顶空固相微萃取（HS-SPME）结合气相色谱-质谱联用（GC-MS）技术对挥发性化合物进行分析。
在液体深层培养的樟芝菌丝体中鉴定出49种挥发性化合物，在固态培养的菌丝体中鉴定出43种挥发性化合物。1-辛烯-3-醇、3-辛酮、1-辛烯-3-基乙酸酯、乙酸辛酯和乙醇是液体深层培养樟芝菌丝体中的主要挥发性化合物，而1-辛烯-3-醇、3-辛酮、3-甲基丁醛、γ-癸内酯和2-糠酸甲酯是固态培养菌丝体中最主要的关键挥发性化合物。
固态培养和液体深层培养樟芝菌丝体中的挥发性化合物相似，但相对含量不同。
