Unidad de Biología Molecular, Institut Pasteur de Montevideo, Montevideo, Uruguay.
Int J Tuberc Lung Dis. 2012 Jul;16(7):947-9. doi: 10.5588/ijtld.11.0559. Epub 2012 Apr 9.
The incidence of tuberculosis (TB) is increasing in high-risk populations in Uruguay, possibly owing to emerging resistance. Mycobacterial interspersed repetitive units (MIRU) genotyping and katG sequence analysis of isoniazid (INH) resistance-associated mutations were performed in 45 INH-resistant Mycobacterium tuberculosis isolates in Uruguayan patients. The genotype distribution among INH-resistant isolates shares features of that of neighbouring countries, with a predominance of Latin American and Mediterranean, T and Haarlem genotypes, although the S genotype was particularly frequent among our isolates. Forty-four per cent of INH-resistant strains harboured the S315T mutation in katG; we found novel katG mutations (W321X, G269T, P232R and G221Wfs1) that could explain INH resistance.
乌拉圭高危人群中的结核病（TB）发病率正在上升，这可能是由于出现了耐药性。对 45 株来自乌拉圭患者的异烟肼（INH）耐药结核分枝杆菌分离株进行了分枝杆菌插入序列重复单位（MIRU）基因分型和 INH 耐药相关突变 katG 序列分析。INH 耐药分离株的基因型分布与邻国具有相似特征，以拉丁美洲和地中海、T 和 Haarlem 基因型为主，但 S 基因型在我们的分离株中尤为常见。44%的 INH 耐药菌株在 katG 中携带 S315T 突变；我们发现了新的 katG 突变（W321X、G269T、P232R 和 G221Wfs1），这些突变可能导致 INH 耐药。
